Untersuchungen zur dendritischen Lokalisierung der arg3.1/Arc mRNA
Final Report Abstract
While Arc/Arg3.1 is essential for the consolidation of synaptic plasticity and memories, little is known about the mechanisms that govern Arc/Arg3.1 mRNA transport and translation in dendrites. To address this issue, we developed the Tri-Hybrid-Method for the in vivo reconstruction of specific RNA-protein interactions. Using this technique in a genetic screen we identified several clones that specifically interact with Arc/Arg3.1 mRNA but not with perikaryal-control RNAs. One of these proteins we named Zinki because it contains a domain of repeated zinc fingers required for the specific binding to Arc/Arg3.1 mRNA. Moreover, Zinki can act as translational repressor in vitro and in vivo. Upon synaptic activity Zinki becomes a substrate for proteosomal degradation. Following dendritic lamina specific stimulation, Zinki vacates those dendritic regions in which translation of the Arc/Arg3.1 mRNA is enhanced indicating that Zinki controls translation of the Arc/Arg3.1 mRNA in this compartment.
Publications
- (2006) Arc/Arg3.1 is essential for the consolidation of synaptic plasticity and memories. Neuron 52:437-444
Plath N, Ghana O, Dammermann B, Errington ML, Schmitz D, Gross C, Mao XS, Engelsberg A, Mahlke C, Weizl H, Kobaz U, Stawrakakis A, Fernandez E, Waltereit R, Bick-Sander A, Therstappen E, Cooke SF, Blanquet V, Wurst W, Salmen B, Boesl MR, Lipp HP, Grant SGN, Bliss TVP, Wolfer DP, Kuhl D
