Untersuchungen zu Struktur-Funktions-Beziehungen der CMP-Sialinsäure-Synthetase unter besonderer Berücksichtigung der Kernlokalisation des Enzyms

• Applicant: Professorin Dr. Rita Gerardy-Schahn
• Subject Area: Microbiology, Virology and Immunology
• Term: from 2000 to 2009
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5236525

• Final Report Year: 2008

Final Report Abstract

No abstract available

Publications

• Molecular cloning of a unique CMP-sialic acid synthetase that effectively utilizes both deaminoneuraminic acid (KDN) and N-acetylneuraminic acid (Neu5Ac) as substrates. Glycobiology 2001; 11: 685-692
  Nakata D., Münster, A.K., Gerardy-Schahn, R., Aoki, N., Matsuda, T., Kitajima, K.
  
• Nuclear localization signal of murine CMP-Neu5Ac synthetase includes residues required for both nuclear targeting and enzymatic activity. J. Biol. Chem. 2002; 277: 19688-19696
  Münster A.K., Weinhold, B., Gotza, B., Mühlenhoff, M., Frosch, M., Gerardy-Schahn, R.
  
• The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase. J. Mol. Biol. 2003; 334: 625-637
  Krapp S., Münster-Kühnel, A.K., Kaiser, J.T., Huber, R., Tiralongo, J., Gerardy-Schahn, R., Jacob, U.
  
• CMP-sialic acid synthetase of the nucleus. Biochim. Biophys. Acta 2004; 1673: 56-65
  Kean E.L., Münster-Kühnel, A.K., Gerardy-Schahn, R.
  
• Structure and function of vertebrate CMP-sialic acid synthetases. Glycobiology 2004; 14: 43R-51R
  Münster-Kühnel A.K., Tiralongo, J., Krapp, S., Weinhold, B., Ritz-Sedlacek, V., Jacob, U., Gerardy-Schahn, R.
  
• Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system. Anal. Biochem. 2005; 337: 12-21
  Fujita A., Sato, C., Münster-Kuhnel, A.K., Gerardy-Schahn, R., Kitajima, K.
  
• Probing a CMP-Kdn synthetase by 1H, 31P, and STD NMR spectroscopy. Biochem. Biophys. Res. Commun. 2005; 327: 565-570
  Haselhorst, T., Münster-Kühnel, A.K., Stolz, A., Oschlies, M., Tiralongo, J., Kitajima, K., Gerardy-Schahn, R., von Itzstein, M.