LC-MS/MS system with high-resolution mass spectrometer and nano-UHPLC - 1/2

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 527318136

Proteomic analysis in translational research has recently gained a lot of momentum. New technologies for sample processing as well as mass spectrometric analysis now enable us to establish novel workflows for a variety of biomedical research applications and even diagnostics. The current instrumentation has been in use for more than 12 years continuously, and the maintenance-related downtime has increased significantly over the past four years. Additionally, it is not competitive any longer, mainly due to slow acquisition and due to the lack of possibilities to perform novel analysis workflows like data-independent mass spectrometry or similar approaches. Therefore, it needs to be replaced with a state-of-the-art instrument. To replace this machine, we request a new instrument. It is planned to be used for various proteomic applications including the analysis of formalin-fixed and paraffine-embedded tissue samples where only limited material is available. Also, the analysis of small samples for relatively small tissue or organoid samples including pull-downs for protein interaction analysis via tagged fusion protein capture and antibody-based pull-downs is increasing and the analysis of body fluids like tear fluid and perilymph needs a highly sensitive and reliable Liquid Chromatography-Mass Spectrometry system with respect to sensitivity, throughput and analytical depth. This requires a device with high scan rates and ultra-high resolution for large sample throughput of very complex as well as very scarce samples. Further, to increase the throughput and reproducibility, the new instrument needs to enable high resolution and high-speed data-independent acquisition and, for validation purposes and to detect specific proteins in complex samples, targeted analysis of specific peptides. Additionally, for separation of the complex mixtures, a ultra-high pressure liquid chromatography system needs to be connected to the mass spectrometer which allows high resolution separation of complex peptide mixtures at nano flow rates. The set-up we are requesting is a combination of a nano ultra-high performance liquid chromatography system, coupled with an ion-mobility unit connected to an ultra-high resolution mass spectrometry machine. This combination is a perfect setup to achieve a precise retention time, limit sample dispersion and system carryover, reduce spectral noise background and deliver sensitive and accurate identification and quantification of peptides at a high resolution.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: LC-MS/MS System mit hochauflösendem Massenspektrometer und nano-UHPLC - Gerät 1 von 2
• Instrumentation Group: 1700 Massenspektrometer
• Applicant Institution: Eberhard Karls Universität Tübingen
• Leader: Professor Dr. Marius Ueffing, Ph.D.