Superoxid als Vermittler einer oxLDL-Wirkung auf Makrophagen

• Applicant: Professor Dr. Bernhard Brüne
• Subject Area: Public Health, Healthcare Research, Social and Occupational Medicine
• Term: from 2001 to 2009
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5311056

• Final Report Year: 2009

Final Report Abstract

Our previous work provided mechanistic insights on inflammatory and survival responses of phagocytes towards atherogenic lipoproteins, particularly oxLDL and PLA-LDL. Additionally, we investigated mechanisms of macrophage polarization and identified PPARy as a key regulatory signaling molecule. Currently we explore macrophage polarization and determine the role of Nrf2 during the macrophage phenotype shift in response to oxLDL.

Publications

• (2006) Oxidized low-density lipoprotein depletes PKCalpha and attenuates reactive oxygen species formation in monocytes/macrophages. Cardiovasc. Res. 7 1 : 574-585
  Kohl, R., Preiss, S., von Knethen, A. and Brune, B.
  
• (2006) Phospholipase A2-modified low-density lipoprotein activates the phosphatidylinositol 3-kinase-Akt pathway and increases cell survival in monocytic cells. Arterioscler. Thromb. Vase. Biol. 26: 2510-2516
  Namgaladze, D, and Brune, B.
  
• (2006) Recognition of apoptotic cells by macrophages activates the peroxisome proliferator-activated receptorgamma and attenuates the oxidative burst. Cell Death Differ. 13: 1533-1540
  Johann, A.M., von Knethen, A., Lndemann, D. and Brune, B.
  
• (2007) Critical role for classical PKC in activating Akt by phospholipase A2-modified LDL in monocytic cells. Cardiovasc. Res. 73: 833-840
  Preiss, S., Namgaladze, D. and Brune, B.
  
• (2007) Peroxisome proliferator-activated receptor y (PPARy) and sepsis. Arch. Immunol. Ther. Exp. 55: 1-7
  von Knethen, A., Soller, M. and Brune, B.
  
• (2007) PPARgamma1 attenuates cytosol to membrane translocation of PKCalpha to desensitize monocytes/macrophages. J. Cell. Biol. 176: 681-694
  von Knethen, A., Soller, M., Tzieply, N., Weigert, A., Johann, A.M., Jennewein, C., Kohl, R. and Brune, B.
  
• (2008) Oxidized LDL attenuates apoptosis in monocytic cells by activating ERK signaling. J. Lipid Res. 49: 58-65
  Namgaladze, D., Kollas, A. and Brune, B.
  
• (2008) Sumoylation of PPARy by apoptotic cells prevents LPS-induced NCoR removal from kB binding sites mediating transrepression of proinflammatory cytokines. J. Immunology 181: 5646-5652
  Jennewein, C., Kuhn, A.-M., Schmidt, M.V., Meilladec-Jullig, V., von Knethen, A., Gonzalez, F. and Brune, B.