Project Details
Einfluss von löslichem P-Selektin auf Hämostase und Thrombose
Applicant
Dr. Wolfgang Bergmeier
Subject Area
Pharmacology
Term
from 2002 to 2005
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5393300
Adhesion receptors expressed on the surface of endothelial cells, platelets, and other hematopoietic cells play an essential role in thrombosis and hemostasis. Some of these receptors are detectable in a soluble form in plasma, but their physiologic function(s) are poorly defined. Soluble P-selectin (sP-sel) was recently shown to induce by enhanced fibrin formation and deposition on growing thrombi. This unexpected function leads to speculations regarding an active role for sP-sel in different clinical complications where increased plasma levels of this molecule have been detected. The studies presented here aim to elucidate the molecular mechanisms involved in sP-sel-induced microparticel formation, to study the role of these microparticels in thrombus formation, and to evaluate possible beneficial effects of sP-sel in clinical bleeding complications. Among others, the methodological approaches include (1) novel flow cytometric assays for studies on mouse blood cells, (2) generation of transgenic mice overexpessing sP-sel, (3) in vitro and ex vivo flow chamber experiments, and in vivo intravital microscopic studies on thrombus formation, and finally (4) clinically relevant mouse models for human bleeding complications.
DFG Programme
Emmy Noether International Fellowships