Chemisch-biologische Hybridverbindungen für die molekulare Organisation, Assemblierung und Manipulation von Proteinen
Zusammenfassung der Projektergebnisse
This project explored how proteins can be organized and controlled in time and in space using chemically defined light-responsive ultasmall elements. By combining synthetic multivalent chelator heads with self-assembling surfaces, we developed molecular nanotools that bind His-tagged proteins with high affinity and specificity. These chemical/biological hybrid systems enable reversible protein immobilization, nanoscale patterning, and photoactivatable interactions under native conditions. Using multivalent interactions and photoactivatable switches, we construted hybrid protein-material interfaces for applications in super-resolution imaging, protein arrays, and the functional control of macromolecular complexes like the proteaseom. This hybrid systems offer precies control over protein function and localzation in two and three dimensions.
Projektbezogene Publikationen (Auswahl)
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(2006) Controlling the activity of the 20S proteasome complex by synthetic gatekeepers. Ängew. Chem. Int. Ed. 45, 5702-5705
Schulze, Katrin; Mulder, Alart; Tinazli, Ali & Tampé, Robert
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(2006) Differential protein assembly on micropatterned surfaces with tailored molecular and surface multivalency. ChemBioChem 7, 1325-1329
Valiokas, Ramūnas; Klenkar, Goran; Tinazli, Ali; Tampé, Robert; Liedberg, Bo & Piehler, Jacob
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(2006) Piezo dispensed density microarrays of multivalent chelating thiols for dissecting complex protein-protein interactions. Änal. Chem. 78, 3643-3650
Klenkar G., Valiokas R., Lundstrom, I., Tinazli A., Tampe, R., Piehler J., Liedberg B.
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(2006) Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation. J. Äm. Chem. Soc. 128, 2365-2372
Lata, Suman; Gavutis, Martynas; Tampé, Robert & Piehler, Jacob
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(2007) Molecular printboards as a general platform for protein immobilization: a supramolecular solution to nonspecific adsorption. Ängew. Chem. Int. Ed. 46, 41044107
Ludden, Manon J. W.; Mulder, Alart; Tampé, Robert; Reinhoudt, David N. & Huskens, Jurriaan
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(2007) Native protein nanolithography that can write, read and erase. Nature Nanotech. 2, 220-225
Tinazli, Ali; Piehler, Jacob; Beuttler, Mirjam; Guckenberger, Reinhard & Tampé, Robert
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(2008) Anchoring of histidine-tagged proteins to molecular printboards: self-assembly, thermodynamic modeling and patterning. Chemistry 14, 2044-2051
Ludden, Manon J. W.; Mulder, Alart; Schulze, Katrin; Subramaniam, Vinod; Tampé, Robert & Huskens, Jurriaan
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(2008) Self-assembled monolayers containing terminal mono-, bis-, and tris-nitrilotriacetic acid groups: characterization and application. Langmuir 24, 49594967
Valiokas, Ramūnas; Klenkar, Goran; Tinazli, Ali; Reichel, Annett; Tampé, Robert; Piehler, Jacob & Liedberg, Bo
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(2008) Conformation of receptor adopted upon interaction with virus revealed by site-specific fluorescence quenchers and FRET-analysis. J. Am. Chem. Soc., 131, 5478-5482
Wruss, Jürgen; Pollheimer, Philipp D.; Meindl, Irene; Reichel, Annett; Schulze, Katrin; Schöfberger, Wolfgang; Piehler, Jacob; Tampé, Robert; Blaas, Dieter & Gruber, Hermann J.
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(2010) Chemical tags mediate the orthogonal self-assembly of DNA strands into supramolecular structures. Small 6, 1732-1735
Mitchell, Nick; Ebner, Andreas; Hinterdorfer, Peter; Tampé, Robert & Howorka, Stefan
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(2010) In situ assembly of macromolecular complexes trigged by light. Proc. NatL Acad. Sei. U.S.A. 107, 6146-6151
Grunwald, Christian; Schulze, Katrin; Reichel, Annett; Weiss, Victor U.; Blaas, Dieter; Piehler, Jacob; Wiesmüller, Karl-Heinz & Tampé, Robert
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(2010) Native laser lithography of His-tagged proteins by uncaging of multivalent chelators. J. Am. Chem. Soc. 132, 5932-5933
Bhagawati, Maniraj; Lata, Suman; Tampé, Robert & Piehler, Jacob
