Molecular and functional profiling of vesicle trafficking pathways in Arabidopsis thaliana

• Applicant: Privatdozent Dr. Joachim Uhrig
• Subject Area: Plant Cell and Developmental Biology
• Term: from 2003 to 2005
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5402546

• Final Report Year: 2007

Final Report Abstract

The project utilized a functional genomics approach to systematically study key players of plant secretion controlling intracellular trafficking, the formation of transport vesicles and their specific delivery to target membranes. Focus has been on the small GTPases of the Rab and ADP Ribosylation Factor (ARF) superfamilies and their cellular partners from Arabidopsis thaliana. In the project part of the co-applicant, the complete family of ARF GTPases has been cloned and initially characterized. A systematic yeast two-hybrid screening approach identified a number of putative downstream effector proteins and revealed a potential role of ARF-GTPases in blue light signalling. Furthermore, an involvement of ARF7 in pollen tube growth and a potential link between ARF8 and pathogen defence has been found.

Publications

• (2004) "ARF-GTPases in plant pathogen interactions". 15th International Conference on Arabidopsis Research, July 11 -14, 2004, Berlin, Germany
  Unte U, Uhrig JF, Schulze-Lefert P, Lipka, V
  
• (2006) "Plant GTPases: regulation of morphogenesis by ROPs and ROS". Curr-Biol 16: R211-213
  Uhrig JF, Hulskamp M
  
• (2006) "Protein interaction networks in plants". Planta, 224, 771-781
  Uhrig JF