Fluorescence Resonance Energy

• Antragsteller: Dr. Jochen Friedrich
• Fachliche Zuordnung: Physikalische Chemie von Molekülen, Flüssigkeiten und Grenzflächen, Biophysikalische Chemie
• Förderung: Förderung von 2003 bis 2004
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5408565

The fluorescence energy transfer of a fluorescence donor group to an acceptor group will be investigated in respect of determining intramolecular distances of gas-phase protein ions. For this purpose a new instrument will be constructed. Ions will be produced by an electrospray source, mass selected by a quadrupole mass filter and stored in a linear quadrupole trap for laser induced fluorescence (LIF) measurement. A Fourier Transform Ion Cyclotron Mass Spectrometer (FTIRCR-MS) allows for high resolution mass analysis. It is planned to use rigid gas-phase polypeptids as model compounds to explore this method. Polypeptides of different length will be labeled with a fluorescent donor (Tryptophan) and acceptor (Dansyl) group and the distance dependence of the fluorescence lifetime of Tryptophan emission ivestigated. In order to allow for future intramolecular distance determination of gas-phase proteins, the Förster distance for this donor/acceptor pair will be determined.

• DFG-Verfahren: Forschungsstipendien
• Internationaler Bezug: USA
• Kooperationspartner: Professor Dr. Alan G. Marshall