Multifunctional molecular structures for biotechnology and clinical diagnostics

• Applicant: Privatdozent Dr. Gerhard Mohr
• Subject Area: Organic Molecular Chemistry - Synthesis and Characterisation
• Term: from 2004 to 2008
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5430298

• Final Report Year: 2008

Final Report Abstract

The main objective of the project was the development of functional materials for the application in biotechnology and in clinical diagnostics. New indicator dyes have been developed that enabled the detection of saccharides in aqueous solution or the determination of ethanol in beer and wine. The optical sensors were also combined with miniaturised sensor modules to develop cheap and small measurement devices. In the framework of the project, the method of molecular imprinting of polymer layers was evaluated. While no clear correlation between imprinting and tailored selectivity could be achieved, new international contacts were established. As a consequence, two EU project applications were successful so that on-going research can be continued. The research in the field of nanomaterials was very successful. New fluorescent nanoparticles for medical research were developed, that enable the continuous monitoring of analyte ions such as chloride, sodium or pH. This is highly relevant for the development of new medication or for the investigation of mechanisms in living cells. This work is also continued within DFG- and EU-projects.

Publications

• G. J. Mohr, New chromogenic and fluorogenic reagents and sensors for neutral and ionic analytes based on covalent bond formation - A review of recent developments, Analytical and Bioanalytical Chemistry, 386 (2006) 1201-214.
  
  
• B. Bussemer, D. Munsel, H. Wünscher, G. J. Mohr, U.-W. Grummt, Electronic properties of neutral dyes in the channels of Zeolite L - A combined spectroscopic and quantum chemical study, Journal of Physical Chemistry B, 111 (2007) 8-15.
  
  
• A. Gräfe, K. Haupt, G. J. Mohr, Optical sensor materials for the detection of amines in organic solvents, Analytica Chimica Acta, 565 (2006) 42-47.
  
  
• D. Römhild, A. Steinke, N. Kirchner, M. Szili, G. J. Mohr, Microoptical remission sensors for aqueous CO2-detection, OPTO 2004, 6th International Conference for Optical Technologies, Optical Sensors and Measuring Techniques, AMA Service, Wunstorf, 2004, Conference Proceedings.
  
  
• G. J. Mohr, B. Bussemer, U.-W. Grummt, Predicting spectral characteristics and reactivity of receptor molecules that perform reversible chemical reactions with analyte molecules, Sensors and Actuators B, 127 (2007) 414-419.
  
  
• G. J. Mohr, Covalent bond formation as an analytical tool to optically detect neutral and anionic analytes, Sensors and Actuators B, 107 (2005) 2-13.
  
  
• G. J. Mohr, Optical sensors for alcohols and amines, Analytix - Fluka, 3 (2006) 6-8.
  
  
• G. J. Mohr, Polymers in Optical Sensors, in Advanced Study Course on Optical Chemical Sensors (Eds. F. Baldini, J. Homola), NATO ASI Series, Kluwer Academic Publishers (2006) 297-321. 3
  
  
• G. J. Mohr, Selected dyes for the preparation of optical chemosensors, Analytix - Fluka, 1 (2007) 18-19.
  
  
• G. J. Mohr, Tailoring the sensitivity and spectral properties of a chromoreactand for the detection of amines and alcohols, Analytica Chimica Acta, 508 (2004) 233-237.
  
  
• G. J. Mohr, U.-W. Grummt, Comparison of trifluoroacetyl monostyryl and distyryl dyes: Effects of chromophore elongation on the spectral properties and chemical reactivity, Journal of Fluorescence, 16 (2006) 185-190.
  
  
• S. M. Korent, A. Lobnik, G. J. Mohr, Sol-gel-based optical sensor for the detection of aqueous amines, Analytical and Bioanalytical Chemistry, 387 (2007) 2863-2870.
  
  
• S. Trupp, A. Schweitzer, G. J. Mohr, A fluorescent water-soluble naphthalimide-based receptor for saccharides with highest sensitivity in the physiological pH range, Organic and Biomolecular Chemistry, 4 (2006) 2965-2968.
  
  
• S. Trupp, A. Schweitzer, G. J. Mohr, Fluororeactands for the detection of saccharides based on hemicyanine dyes with a boronic acid receptor, Microchimica Acta, 153 (2006) 127-131.
  
  
• T. Carofiglio, C. Fregonese, G. J. Mohr, F. Rastrelli, U. Tonellato, Optical sensor arrays: one-pot, multiparallel synthesis and cellulose immobilization of pH and metal ion sensitive azo-dyes, Tetrahedron, 62 (2006) 1502-1507.