Static and dynamic cultivation conditions to characterise matrix qualities of simple and complex polySia-based materials. Monitoring of cell parameters by DNA microarray and cell biological methods
Final Report Abstract
Samples from work package WP 2.3, 2.4. and 2.5 were obtained and initially tested according to a pre-screening scheme under static conditions (phase 1). Furthermore, model matrices (ceramic, collagen, microspheres) were included to the screening. Materials found to be suitable for cell culture applications were subjected to cultivation under dynamic conditions (phase 2) in specialized bioreactors developed in this work package WP 2.6. The screening system for biocompatibility testing was successfully optimized by the development and application of different bioreactor systems based on perfusion mode performance. Three specialized bioreactors were developed to meet the different biomaterial characteristics such as porosity and stiffness. All bioreactors are suitable for parallel testing of several probes and designed for e.g. integration of sensors and enable automation. Thus, fast and reproducible testing of samples can be performed under controlled conditions. The polySia based materials delivered to the Institut für Neuroanatomie were analysed with regard to their impact on and interaction with neonatal Schwann cells. These easy to handle cell culture model revealed that the polySia-based or coated materials did not allow for sufficient cell adhesion and survival in vitro. Nevertheless, some of the materials were further evaluated in short term in vivo experiments as reported for WP 2.7.
Publications
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(2008). Synthesis and biological evaluation of a polysialic acid-based hydrogel as enzymatically degradable scaffold material for tissue engineering. Biomacromolecules., 9, 2353-2359
Berski, S., van Bergeijk, J., Schwarzer, D., Stark, Y., Kasper, C., Scheper, T., Grothe, C., Gerardy-Schahn, R., Kirschning, A., and Drager, G.
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(2010). Polysialic acid immobilized on silanized glass surfaces: a test case for its use as a biomaterial for nerve regeneration. J. Mater. Sci. Mater. Med., 21, 1371-1378
Steinhaus, S., Stark, Y., Bruns, S., Haile, Y., Scheper, T., Grothe, C., and Behrens, P.
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(2010). Synthesis of new polysialic acid derivatives. Macromol. Biosci., 10, 1028-1033
Su, Y., Kasper, C., Kirschning, A., Dräger, G., and Berski, S.
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Development of a screening system for parallel testing of biomaterials under static and dynamic conditions; 4th Biomaterial Symposium Vienna 2010
S. Kress, Neumann, A, S. Böhm, T. Hatlapatka, Kasper, C.
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Immobilization of polysialic acid on nanoporous silica particles Sialoglyco 2010
S. Williams, I. Bremer, A. Christel, S. Böhm, C. Kasper, Y. Su, G. Dräger, A. Kirschning, R. Gerardy-Schahn, P. Behrens
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Immobilization of polysialic acid on nanoporous silica particles. DGMB 2010
S. Williams, I. Bremer, A. Christel, S. Böhm, C. Kasper, T. Scheper, R. Gerardy-Schahn, P. Behrens
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Nanoparticles for Multiple Functionalization of Scaffolds for Tissue Engineering Applications, Hybrid Materials 2011
A. Christel, A. Neumann, S. Williams, A. Hoffmann, C. Kasper, P. Behrens
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Nanoporous silica nanoparticles: Strategies for functionalization and cell culture tests; DGMB 2011
S. Williams; A. Neumann; A. Christel; S. Böhm; C. Kasper; P. Behrens
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Polysaccharide –based biomaterials for Tissue Engineering; 24th European Conference on Biomaterials, ECB 2011
A. Neumann, I. Bice, T. Scheper, C. Kasper
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Evaluation of Biocompatibilty of Silica Nanoparticles in vitro using Bioreactors; 5th Biomaterial Symposium Vienna 2012
Neumann, A, Strauß, C., Christel, A., Williams, S., Behrens, P., Kasper, C.
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Polysialic acid immobilized on nanoporous silica nanoparticles: Strategies for functionalization and cell culture tests; NanoBioMed 2012
S. Williams; A. Neumann; A. Christel; S. Böhm; C. Kasper; P. Behrens
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(2013) BMP2-loaded nanoporous silica nanoparticles promote osteogenic differentiation of human mesenchymal stem cells. RSC Adv. 3, 24222 – 24230
Neumann, A., Christel, A., Kasper, C., and Behrens, P.
