Structural studies on factor assisted protein folding and targeting

• Applicant: Professorin Paola Fucini, Ph.D.
• Subject Area: Structural Biology
• Term: from 2005 to 2011
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5442577

• Final Report Year: 2015

No abstract available

Publications

• (2008) The Oxazolidinone Antibiotics perturb the Ribosomal Peptidyl-transferase Center and Effect tRNA Positioning. PNAS 105: 13339-13344
  Wilson D.N., Schluenzen F., Harms J.M., Starosta A.L., Connell S.R. and Fucini P.
  
• (2008) Translational regulation via L11: Molecular switches on the ribosome turned on and off by thiostrepton and micrococcin. Mol Cell, 30: 26-38
  Harms J.M., Wilson D.N., Schluenzen F., Connell S.R., Stachelhaus T., Zaborowska Z., Spahn C.M.T. and Fucini P.
  
• (2008). A new tRNA-intermediate revealed on the ribosome during EF4-mediated backtranslocation. NSMB 15: 910-915
  Connell S.R., Topf M., Qin Y., Wilson D.N., Mielke T., Fucini P., Nierhaus K. and Spahn C.M.T.
  
• (2009) Probing Protein Folding on the Ribosome by Solution State NMR Spectroscopy. Journal of Biomolecular Structure and Dynamic 26: 846-846
  Hsu STD, Cabrita LD, Fucini P, Christodoulou J, Dobson CM
  
• (2009) Probing Side-Chain Dynamics of a Ribosome-Bound Nascent Chain Using Methyl NMR Spectroscopy. J Am Chem Soc 131: 8366-
  Hsu STD, Cabrita LD, Fucini P, Christodoulou J, Dobson CM
  
• 2009) Structure, Dynamics and Folding of an Immunoglobulin Domain of the Gelation Factor (ABP-120) from Dictyostelium discoideum. J Mol Biol 388: 865-879
  Hsu STD, Cabrita LD, Fucini P, Dobson CM, Christodoulou J
  
• (2010) Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites. Nature 468, 713-6
  Ratje, A.H., Loerke, J., Mikolajka, A., Brunner, M., Hildebrand, P.W., Starosta, A.L., Donhofer, A., Connell, S.R., Fucini, P., Mielke, T., Whitford, P.C., Onuchic, J.N., Yu, Y., Sanbonmatsu, K.Y., Hartmann, R.K., Penczek, P.A., Wilson, D.N. & Spahn, C.M.
  
• (2010) PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the ribosome-recycling factor (RRF) and elongation factor G (EF-G) J Biol Chem 285: 4006-14
  Sharma M.R., Dönhöfer A., Barat C., Marquez V., Datta P.P., Fucini P., Wilson D.N., Agrawal R.K.
  
• (2013) Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology. J Biomol NMR. 56: 85-93
  Gelis, Ioannis; Vitzthum, Veronika; Dhimole, Neha; Caporini, Marc A.; Schedlbauer, Andreas; Carnevale, Diego; Connell, Sean R.; Fucini, Paola & Bodenhausen, Geoffrey
  
• Structural Characterization of an Alternative Mode of Tigecycline Binding to the Bacterial Ribosome. Antimicrobial Agents and Chemotherapy, Vol 59, No 5, May 2015, pp. 2849–2854
  Schedlbauer, Andreas; Kaminishi, Tatsuya; Ochoa-Lizarralde, Borja; Dhimole, Neha; Zhou, Shu; López-Alonso, Jorge P.; Connell, Sean R. & Fucini, Paola