Regulation of alternative polyadenylation by RNA-binding proteins in senescence (A06)

• Subject Area: Biochemistry ; Cell Biology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 520471345

Alternative polyadenylation (APA) is an RNA processing mechanism that generates transcripts with different 3′-ends for the same gene. Recent studies have shown that APA contributes to cellular senescence through its influence on gene expression. We will investigate whether APA undergoes upstream (i.e., changes in splicing or transcription) or intrinsic (i.e. changes in the APA machinery) loss of fidelity associated with cellular senescence by combining poly(A) sequencing with eCLIP of key regulatory proteins and mapping of their protein-protein interactions to identify generalizable points of intervention.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1678: Systems-level consequences of fidelity changes in mRNA and protein biosynthesis
• Applicant Institution: Universität zu Köln
• Project Head: Dr. Ina Huppertz