High resolution mass spectrometer with coupled nanoUHPLC system for proteomics

• Subject Area: Plant Sciences
• Term: Funded in 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 544805865

The MSBioLMU has been registered as a core facility in the DFG database since 2014 (Resource Identifier: RI_00365) and provides proteomics and metabolomics services predominantly to the Faculty of Biology, with a particular emphasis on plant sciences. Advances in mass spectrometry techniques for quantitative analyses of complex protein profiles have been significant in recent years, leading to the decision to replace the current 9-year old mass spectrometer used for proteomics. In addition to routine analyses, there is a growing focus on quantitative shotgun proteome analyses, especially those that consider post-translational modifications of plant proteins, particularly in relation to the chloroplast's role in adapting to changing environmental conditions. The acquisition of a new high-resolution mass spectrometer with a nanoUHPLC system will empower the MSBioLMU core facility to maintain its status as a high-performance service unit for the Faculty of Biology. The mass spectrometer currently in use no longer meets contemporary technical standards and age-related increased downtime is expected. The mass spectrometry service requires technically state-of-the-art instruments with maximum runtime. With the recent increase in sample numbers, there is a pressing need not only to comprehensively analyse highly complex protein mixtures but also to ensure high sample throughput rates. Consequently, we are requesting an ion mobility mass spectrometer able to perform high speed data independent acquisition with correspondingly highest sensitivity and a large dynamic range.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Hochauflösendes Massenspektrometer mit gekoppeltem nanoUHPLC-System für die Proteomik
• Instrumentation Group: 1700 Massenspektrometer
• Applicant Institution: Ludwig-Maximilians-Universität München
• Leader: Professor Dr. Dario Leister