Quantitative cytogenetic characterization and structural analysis of REC8 proteoforms in plant meiosis (A04)

• Subject Area: Plant Genetics and Genomics ; Biochemistry ; Plant Biochemistry and Biophysics ; Bioinformatics and Theoretical Biology ; Biophysics ; Evolution and Systematics of Plants and Fungi ; Plant Physiology ; Structural Biology ; Plant Cell and Developmental Biology ; Cell Biology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 514901783

A04 investigates the interaction of protein complexes regulating meiotic recombination, focusing on REC8-cohesin complexes in barley. REC8.2, one of two copies in cereal genomes, influences recombination rate variation. Using wild barley lines, this project examines the impact of allelic variation on recombination frequencies through cytological and biochemical/biophysical methods, including cryo-EM. The aim is the elucidation of binding specificities within the REC8-cohesin complex and how these are affected by different proteoforms.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1664: Plant Proteoform Diversity - SNP2Prot
• Applicant Institution: Martin-Luther-Universität Halle-Wittenberg
• Project Heads: Dr. Steven Dreissig; Professor Dr. Panagiotis L. Kastritis