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The functional role of protein O-mannosylation

Fachliche Zuordnung Zellbiologie
Förderung Förderung von 2007 bis 2011
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 54554815
 
Protein O-mannosylation represents an evolutionarily conserved, essential post-translational modification with immense impact on a variety of cellular processes. In humans, defects in O-mannosylation lead to Walker-Warburg syndrome, a severe congenital muscular dystrophy associated with defects in neuronal migration. In mouse and yeasts, loss of O-mannosylation causes lethality. In yeasts/fungi, O-mannosylation is indispensable for cell wall integrity, cell morphogenesis and maintenance of cell polarity, however, the molecular basis is only very poorly understood. We want to elucidate the molecular function of O-mannosyl glycans synthesized by different protein Omannosyltransferase complexes and examine the role of O-mannosylation in cell wall integrity, morphogenesis and cell polarity. Using fission yeast as model organism, we will combine classical genetic methods and genomics with molecular, cell biological and biochemical methods to identify and characterize O-mannosylated proteins that are important for viability of yeast. In particular, we will focus on proteins that are vital for cell wall integrity and cell polarity. Further, our analyses will identify components and pathways that compensate the lack of distinct protein Omannosyltransferases. Our studies will significantly improve our understanding of protein O-mannosylation and identify new O-mannosylated proteins which are likely to be crucial for general aspects of fungal growth and development.
DFG-Verfahren Sachbeihilfen
 
 

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