Structure-function analysis of lipid remodelling in tail-anchored protein insertion into the ER membrane (P04)

• Subject Area: Structural Biology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 511488495

The project will delineate the structural and functional implications of lipid-protein interactions for TA protein insertion and membrane morphology at the ER membrane. We will combine structural studies using cryo-EM single particle analyses of distinct functional states of the GET insertase with TA insertion studies and multiscale molecular dynamics simulations. Thereby, we will dissect the dynamic interplay between the GET machinery, membrane lipids, and TA substrates to understand the molecular mechanisms of TA protein insertion.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1638: Cellular membrane remodelling – how changing form creates function
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Head: Professorin Dr. Irmgard Sinning