PinkyCaMP- new red-shifted genetically encoded calcium sensors

• Applicant: Professorin Dr. Olivia Andrea Masseck
• Subject Area: Cognitive, Systems and Behavioural Neurobiology ; Experimental and Theoretical Network Neuroscience
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 548214625

We want to overcome current technical and methodological limitations and develop improved red-shifted calcium sensors to expand the available toolbox for combined imaging and optogenetic approaches. We will utilize PinkyCaMP3.0 (as this variant already has an increased brightness) as starting point for the development of genetically encoded calcium sensors that will outperform existing red-shifted calcium sensors to readout neuronal activity. These red-shifted Ca2+ sensors will be advantageous when it comes to experiments in which fluorescent sensors are combined with optogenetics, biosensors for neurotransmitters or other imaging approaches.

• DFG Programme: Research Grants
• Co-Investigator: Professor Dr. Andreas Reiner