Dynamic epigenomics and lipidomic signatures of human MASLD (A01)

• Subject Area: Gastroenterology
• Term: since 2025
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 535081457

We will perform a multidimensional characterization of human liver biopsies using single nuclei RNA and epigenetic technologies and combine them with spatial functional genomic and lipidomic technologies and RNA velocity analysis. Clinically, we will analyze both cross-sectional samples of disease strata from normal controls to MASH fibrosis and paired longitudinal samples of patients before and after bariatric surgery. Our findings will allow an unbiased global assessment of cell compartments and pathways in human MASLD and help to identify clinically relevant molecular drivers for MASLD outcome, allowing further in-depth investigation in this CRC/TR.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 412: Metabolic dysfunction-associated steatotic liver disease: Translating mechanisms to tailored therapeutic concepts
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Project Heads: Professor Dr. Jochen Hampe; Professor Dr. Andrej Shevchenko