Development of an in vitro platform to study genetic mechanisms driving MASLD/MASH progression (A03)

• Subject Area: Gastroenterology
• Term: since 2025
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 535081457

Project A03 uses multicellular hepatic organoid models derived from human-induced pluripotent stem cells (hiPSCs) to develop an in vitro platform for investigating genetic mechanisms driving MASLD/MASH progression. Using CRISPR-based genetic screens and advanced co-culture models, the project will analyze lipid metabolism, lipotoxicity, and protective networks in hepatocytes. The findings are expected to reveal new therapeutic targets to modulate inflammatory processes and enhance liver regeneration.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 412: Metabolic dysfunction-associated steatotic liver disease: Translating mechanisms to tailored therapeutic concepts
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Project Heads: Dr. Milad Rezvani; Professor Ludovic Vallier, Ph.D.