Project Details
Projekt
High resolution LC-MS system
Subject Area
Agriculture, Forestry and Veterinary Medicine
Term
Funded in 2025
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 564759822
The applicants, a group of crop science professors at Justus Liebig University Giessen, want to use the intended high resolution LC-MS system for gaining insights into the plant metabolome, into patterns in the concentration of phytohormones or into specific questions involving secondary metabolites. These data sets will be used to precisely characterize tolerance strategies against abiotic and biotic stress in different crops (among which faba bean, rapeseed, sorghum and wheat) and to relate them with other 'omics' data sets like sequence variation, gene activity or epigenetic data. The higher aim of these analysis is to increase selection accuracy in plant breeding and therefore to accelerate the development of climate-resilient crops. The majority of the intended research questions deals with variation in metabolites, phytohormones and secondary metabolites in different genotypes, treatments or time points, which means with the comparison of substances between groups. Therefore, we generally need both non-targeted approaches for characterisation of complex plant extracts as well as targeted approaches for high throughput screening of selected metabolites. In most projects, we would therefore first analyze contrasting samples in respect to genetic background, tissue or treatment in a non-targeted way to identify compounds which differentiate between the samples. If the research question allows, those data can be used to select specific compunds to be screened in a targeted way in large populations. Like this, we are able to represent both the metabolic diversity and the genetic variation in the large populations necessary for plant breeding. Therefore, we apply for a high resolution LC-MS system including a UHPLC component, which offers high throughput both for non-targeted and for targeted approaches. The technology of choice is quadrupole time of flight (Qtof) allowing to generate highly resolved MS/MS spectra to identify unknown compounds.
DFG Programme
Major Research Instrumentation
Major Instrumentation
Hochauflösendes LC-MS-System
Instrumentation Group
1700 Massenspektrometer
Applicant Institution
Justus-Liebig-Universität Gießen
