Project Details
Projekt
High troughput-microscopy system
Subject Area
Medicine
Term
Funded in 2025
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 564856936
Multiplexing (parallel detection of multiple parameters on the same tissue section) established in histopathology in the recent years as an important method. This method allows on the one hand deep phenotyping and on the other hand spatial resolution. Since 2016, iPATH.Berlin as core unit of the Charité-Universitätsmedizin Berlin offers multiplexing of 7 parameters. For this, the Vectra3 microscopy system is used. Vectra3 works with spectral unmixing. The advantage of multispectral micrscopy is the opportunity to multiplex in high resolution under optimal conditions for the primary antibodies. For detection, fluorochromes with overlapping spectra can be used as well as chromogens that can not be distinguished with the human eye in coexpression. The camera records the spectra from 420 to 720 nm, that are spectrally unmixed during analysis. This system was improved and developed further. The improved system is now adapted for high-troughput performance. Additionally, it allows for multiplexing with 9 parameters and on-board unmixing (spectral unmixing occurs already during scanning). Simultaneously, the auto-fluorescence is detected and eliminated, improving the signal-to-noise ratio. The main advantage of this micrscopy system is the cyclic staining. Here, the primary antibodies are incubated under optimal conditions and signals are enhanced via the detection system. Additionally, the imaging occurs only once (all colours are detected at the same time). This implies that no image series have to be aligned. Additionally, this high-throughput microscopy system offers the opportunity of multiomic analyses e.g., the simultaneous detection of mRNA and protein on the same tissue section. This allows for a comprehensive approach on various molecular levels. This high-throughput microscopy system works in brightfield but also in fluorescence and allows for the use of established protocols and analyses by iPATH.Berlin and the users. iPATH.Berlin applies for the high-throughput microscopy system to offer an improved multiplexing technology including shorter processing time at the Charité-Universitätsmedizin Berlin to all co-applicants and interested research groups in a professional manner.
DFG Programme
Major Research Instrumentation
Major Instrumentation
Hochdurchsatz-Multispektrales Mikroskopsystem
Instrumentation Group
5042 Mikroskope für Hochdurchsatz und Screening
Applicant Institution
Charité - Universitätsmedizin Berlin
