Intracellular transport of the internal virion components of influenza A virus to the assembly site
Final Report Abstract
Influenza viruses have caused devastating pandemics and multiple epidemics in the past, and continue to be a major health problem and huge economic burden worldwide. Thus, it is important to understand the characteristics of influenza viruses and to elucidate the extensive interplay between virus and host. My work has focused on the identification and characterization of human host cell factors which are required for influenza virus replication. Using a genome-wide RNAi screen we identified 295 of these factors. Bioinformatic analyses of our results highlighted several cellular pathways, such as kinase-mediated signalling or the ubiquitin pathway, that are of particular importance for the virus. For a subset of these host cell factors we characterized their function in the viral replication cycle. Furthermore, we show that compounds which target the identified host proteins inhibit virus replication, thus opening up the possibility of developing a new class of anti-influenza virus drugs.
Publications
- The role of calcium and sodium ion currents in influenza A virus replication. American Society of Virology meeting, Ithaca, NY, U.S.; July 2008
Stertz S, Hoffmann H-H, Rohatgi R, Satlin LM, Palese P, Shaw ML
- A genome-wide RNAi approach to identify and characterize host cell factors involved in influenza virus replication. 5th Orthomyxovirus Research Conference, Freiburg, Germany; September 2009
Stertz S, König R, de Jesus P, Alamares J, Young JAT, García-Sastre A, Palese P, Chanda SK, Shaw ML
- Generation of influenza A virus polymerase specific monoclonal antibodies poster presentation at the Centers of Excellence for Influenza Research and Surveillance, Minneapolis, MN, U.S.; June 2009
Stertz S, Ngai L, Varble A, Petersen E, Moran T, García-Sastre A, Palese P
- Human Host Factors Required for Influenza Virus Replication. 17th Symposium on Virus-Host Interactions New York, NY, U.S.; December 2009
Stertz S, König R, Zhou Y, Inoue A, Hoffmann H-H, Bhattacharyya S, Alamares J, Tscherne DM, Ortigoza MB, Liang Y, Gao Q, Andrews SE, Banyopadhyay S, De Jesus P, Tu BP, Pache L, Shih C, Orth A, Bonamy G, Miraglia L, Ideker T, García-Sastre A, Young JAT, Palese P, Chanda SK, Shaw ML
- Human Host Factors Required for Influenza Virus Replication. Keystone meeting on Virus Entry and Pathogenesis Taos, NM, U.S.; February 2010
Stertz S, König R, Zhou Y, Inoue A, Hoffmann H-H, Bhattacharyya S, Alamares J, Tscherne DM, Ortigoza MB, Liang Y, Gao Q, Andrews SE, Banyopadhyay S, De Jesus P, Tu BP, Pache L, Shih C, Orth A, Bonamy G, Miraglia L, Ideker T, García-Sastre A, Young JAT, Palese P, Chanda SK, Shaw ML
- Human Host Factors Required for Influenza Virus Replication. Nature. 2010 Feb 11;463(7282):813-7
König R, Stertz S, Zhou Y, Inoue A, Hoffmann H-H, Bhattacharyya S, Alamares J, Tscherne DM, Ortigoza MB, Liang Y, Gao Q, Andrews SE, Banyopadhyay S, De Jesus P, Tu BP, Pache L, Shih C, Orth A, Bonamy G, Miraglia L, Ideker T, García-Sastre A, Young JAT, Palese P, Shaw ML, Chanda SK
- Protection of mice against lethal challenge with 2009 H1N1 influenza A virus by 1918-like and classical swine H1N1 based vaccines PLoS Pathogens. 2010 Jan 29;6(1):e1000745
Manicassamy B, Medina RA, Hai R, Tsibane T, Stertz S, Nistal-Villán E, Palese P, Basler CF, García-Sastre A
