FOR 1068:  From Chromatin to Ribosomes: Regulation and Mechanisms of Ribosome Biogenesis

• Subject Area: Biology
• Term: from 2008 to 2011
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 62891577

Ribosome biogenesis is a fundamental and very complex process in all living cells. Formation of the two eukaryotic ribosomal subunits requires all three nuclear transcription machineries, four ribosomal RNAs, more than 70 ribosomal proteins, more than 150 non-ribosomal factors and about 100 non-coding small RNAs. The huge efficiency of ribosome biogenesis - a growing yeast cell produces more than 40 ribosomes per second - demands an optimal coordination of all participating processes and a major amount of cellular energy. Therefore, regulation of ribosome biosynthesis is tightly regulated.
The long-term goal of the Research Unit is to determine the structural basis of the machineries involved and to dissolve and to connect single steps of ribosome biosynthesis. Thus, it should be possible to gain a comprehensive view, how the complex processes leading to mature ribosomes are regulated and coupled. Our Research Unit opens the particular perspective to study the coupling of chromatin structure, transcription, maturation and transport of ribonucleoprotein complexes (RNP) using the biogenesis of ribosomes.

• DFG Programme: Research Units

Projects

• Analyse der Funktion und Assemblierung eukaryotischer ribosomaler Proteine (Applicant Milkereit, Philipp )
• Analyse der Komposition und postranslationalen Modifikationen von Chromatin am ribosomalen DNA Lokus in verschiedenen transkriptionellen Zuständen (Applicant Griesenbeck, Joachim )
• Bildung transkriptionsaktiver RNA Polymerase I-Komplexe in der Hefe (Applicant Tschochner, Herbert )
• Die Funktion heterodimerer Noc-Komplexe und anderer nichtribosomaler Proteinmodule bei der Reifung von eukaryotischen Präribosomen (Applicant Tschochner, Herbert )
• Koordination der Forschungsgruppe 1068 (Applicant Tschochner, Herbert )
• Massenspektrometrische Analyse von Proteinen (Applicant Deutzmann, Rainer )
• Nukleosomen Positionierung durch Chromatin Remodeling Komplexe: Existenz und molekulare Grundlagen eines "Remodeler Codes" (Applicant Längst, Gernot M. )
• Regulation und Koordination von Transkription und der Prozessierung am rRNA Gen (Applicant Längst, Gernot M. )
• Strukturanalyse der RNA-Polymerase I und eines minimalen RNA-Polymerase-Initiationskomplexes (Applicant Cramer, Patrick )
• Wechselwirkungen zwischen Domänen der archaeellen Transkriptionsmaschinerie und Vergleich mit dem eukaryotischen System (Applicant Thomm, Michael )

• Spokesperson: Professor Dr. Herbert Tschochner