Project Details
Projekt
Calcium network control of nutrient and metabolite transport
Applicant
Professor Dr. Rainer Hedrich
Subject Area
Plant Cell and Developmental Biology
Term
from 2008 to 2017
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 49476430
The picture of fast ABA-signalling leading to the Ca2+-dependent activation of S-type anion channels in guard cells is quite detailed by now. However, the majority of collected data is of a qualitative nature and therefore not sufficient to mathematically model fast ABA-signalling in guard cells so far.Thus within the proposed project we intend to i) decompose and quantify the calcium signal itself, ii) the activity and affinity of signal-decoding protein kinases, the iii) abundance of and stoichiometry between ABA-signalling components. Furthermore, we will address the following key questions: iv) How do the individual signalling components influence each other? v) What are the spatio-temporal events that occur during ABA-dependent closure? vi) How do kinases with different Ca2+ affinities encode Ca2+ signals and thereby fine-tune the activity of anion channels? The collection of these quantitative data together with mathematical modeling will proof or disproof our current notion of fast ABA-signalling in guard cells and will come up with new predictions and in turn experiments on ABA-induced stomatal closure.
DFG Programme
Research Units
