Project Details
Projekt
Biochemical regulation and function of CDPKs in the calcium-regulated abiotic stress signalling network
Applicant
Professorin Dr. Tina Romeis
Subject Area
Plant Cell and Developmental Biology
Term
from 2008 to 2017
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 49476430
Calcium-dependent protein kinases (CDPK) are serine/threonine protein kinases in which the calcium-binding sensor domain is directly joined to a protein kinase effector domain within one molecule. Our objective is to investigate the contribution of CPK21 and CPK23 in the intracellular calcium-regulatory network during the onset of early abiotic stress responses. In part 1, we will perform a comparative analysis of CPK21 and CPK23 in vivo function and biochemical activation by phosphorylation and calcium-binding, and the integration of CPK21 and CPK23 into ABA-perception signalling complexes. In part 2 we will address the CDPK signalling network: this includes the identification of potential phosphorylation targets among ion channels, transporters or members of the RCAR family and the co-regulation with further members of the CDPK or CIPK class of protein kinases or phosphatases in the context of abiotic stress.
DFG Programme
Research Units
