FACS-based cell isolation and clinical translation ((4)-Q03)

• Subject Area: Gastroenterology
• Term: from 2009 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 36842431

The Q3 Core Unit provides the projects with reliably, standardized and efficiently isolated primary cells from liver and kidney from mice and rats. We have established FACS-based protocols for isolating hepatic stellate cells, sinusoidal endothelium as well as renal podocytes and parietal cells. We are currently extending our protocols to human cell types for translational projects.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 57: Organ Fibrosis: From Mechanisms of Injury to Modulation of Disease
• Major Instrumentation: Single Cell Solution Controller
• Instrumentation Group: 3150 DNA-Sequenzer
• Applicant Institution: Rheinisch-Westfälische Technische Hochschule Aachen
• Project Heads: Professor Frank Tacke, Ph.D., until 3/2019; Professor Dr. Ralf Weiskirchen