The role of topological events for meiotic homologue paring and genome haploidization

• Antragsteller: Professor Dr. Harry Scherthan, seit 4/2010
• Fachliche Zuordnung: Zellbiologie
• Förderung: Förderung von 2009 bis 2015
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 114974210

Genome reduction by meiosis requires the pairing and separation of homologous chromosomes during meiosis I, eventually leading to genome reduction to the haploid complement in sperm or spores. Breaks in double stranded DNA (DSBs) and their ordered repair is required to pair homologues in prophase I of most eukaryotes. The homologue search process poses a three-dimensional problem o the cell and involves chf-omosome mobility in mammals and yeasts, with the latter organism displaying dramatic movements throughout prophase I. It has been hypothesized that chromosome mobility contributes to the disruption of excess recombinogenic chromosome interactions, but this issue remains poorly understood. Any pre-existing 3D order in the premeiotic nucleus will support homology search. Hence, pair-wise centromere associations have been considered to facilitate homologue sorting in plants and yeast. In this proposal we will investigate /) parental genome distribution and centromere associations in meiosis of mouse M. spretus x M. musculus hybrids and //) whether chromosome mobility in yeast contributes to elimination of ectopic recombinogenic interactions instigated by ionising radiation of prophase I cells. /'//) Finally, the genetic interaction of mutations that affect telomere clustering will be probed in strains with lasting telomere clustering.

• DFG-Verfahren: Schwerpunktprogramme
• Teilprojekt zu: SPP 1384: Mechanisms of Genome Haploidization
• Großgeräte: Beleuchtungseinheit zur Fluoreszenzanregung
• Gerätegruppe: 5040 Spezielle Mikroskope (außer 500-503)
• Ehemaliger Antragsteller: Professor Dr. Roland Kappler, von 3/2010 bis 4/2010