Nanoscale regulation of small GTPase switches at the trans-Golgi network/recycling endosomal interface (A20)

• Subject Area: Biochemistry
• Term: since 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 278001972

Polarized sorting of receptors and signaling molecules is essential for cellular homeostasis in epithelial cells. In project A20 N we will investigate the role of switches of the ARF family of GTPases in polarized post-Golgi trafficking. Taking advantage of live-cell super-resolution imaging, CRISPR/Cas9 gene editing and mass-spectrometry we aim at dissecting the role of the various ARFs in post-Golgi sorting and their role in communication between the closely juxtaposed trans-Golgi network (TGN) and recycling endosomal compartments.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 186: Molecular Switches: Spatio-temporal Control of Cellular Signal Transmission
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Head: Professorin Dr. Francesca Bottanelli, Ph.D.