3D Bio-Strukturierung mithilfe der selbst-faltenden Polymerschichten

• Antragstellerin: Privatdozentin Dr. Alla Synytska, seit 8/2015
• Fachliche Zuordnung: Experimentelle und Theoretische Polymerphysik ; Physikalische Chemie von Festkörpern und Oberflächen, Materialcharakterisierung
• Förderung: Förderung von 2009 bis 2020
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 114626003

• Erstellungsjahr: 2021

Zusammenfassung der Projektergebnisse

In summary, within the first funding period, we successfully developed novel synthetic routes for synthesis of thermoresponsive surfaces and applied them for control of adsorption of proteins and for smart patterning of proteins using visible light. We made substantial progress in development of new kinds of stimuli-responsive surfaces based on grafted polymer layers, adsorbed polymer monolayers, fusible materials and hydrogel films with different switching mechanism. We in detail investigated mechanism of switching of polymer brushes and the effect of their architecture on the switching behavior. We demonstrated new possibilities for patterning of proteins, particles and liquids. We investigated possibilities and limitations of reversible surface patterning of proteins using photothermal approach. It was shown that resolution down to 5 um could be achieved practically and theory predicts that higher resolution is possible when more powerful lasers are used. Another considerable achievement of the project is development of new method for 3D microfabrication using spontaneous folding of thin polymer bilayer films. In the second funding period we developed a cordially new approach for fabrication of complex 3D cellular structure using shape transformation – 4D biofabrication. The approach opens new perspective for fabrication of vascular structures as well as tissues with uniaxial orientation of cells (for example muscle tissue) that is now explored in the frames of new DFG projects.

Projektbezogene Publikationen (Auswahl)

• Protein-resistant polymer coatings based on surface-adsorbed poly(aminoethyl methacrylate)/poly(ethylene glycol) copolymers Biomacromolecules 2010, 11 (1), 233–237
  Ionov, L.; Synytska, A.; Kaul, E.; Diez, S.
  
• Mixed Polymer Brushes with Locking Switching ACS Appl. Mater. & Interfaces, 2012, 4 (1), 483–489
  Ionov, L.; Minko S.
  
• Hierarchical multi-step folding of polymer bilayers Adv.Funct. Mater. 2013, 23, 2295–2300
  Stoychev, G.; Turcaud, S.; Dunlop, J.; Ionov, L.
  
• Biodegradable self-folding polymer films with controlled thermo-triggered folding, Adv. Funct. Mater. 2014, 24(27), 4357–4363
  Stroganov, V.; Zakharchenko, S.; Sperling, E.; Meyer, A.K.; Schmidt, O.G; Ionov, L.
  
• Programmable patterning of protein bioactivity by visible light, Nano Letters 2014, 14 (7), 4050–4057
  Reuther, C.; Tucker, R.; Ionov, L.; Diez, S.
  
• Stimuli-Responsive Microjets with Reconfigurable Shape Angew. Chem. Int. Ed. 2014, 126(10), 2711
  Magdanz, V.; Stoychey, G.; Ionov, L..; Sanchez, S.; Schmidt, O.
  
• 4D Biofabrication using Shape-Morphing Hydrogels Adv. Mater. 2017 29, 170344
  Kirillova, A.; Maxson, J.; Gomillion, C.T.; Ionov, L.
  
• 4D biofabrication: 3D cell patterning using shape-changing films Adv. Funct. Mater. 2018, 28 1706248
  Stroganov, V.; Pant, K.; Stoychev, G.; Janke, A.; Jehnichen, D.; Fery, A.; Handa, H.; Ionov, L.
  
• Effect of Architecture of Thermoresponsive Copolymer Brushes on Switching of Their Adsorption Properties Macromol. Chem. Phys. 2019, 220, 1900030
  Marschelke, C.; Puretskiy, N.; Raguzin, I.; Melnyk, I.; Ionov, L.; Synytska, A.
  
• 4D biofabrication of fibrous artificial nerve graft for neuron regeneration Biofabrication 2020 12 035027
  Apsite, I.; Constante, G.; Dulle, M.; Vogt, L.; Caspari, A.; Boccaccini, A.; Synytska, A.; Salehi, S.; Ionov, L.
  
• 4D Biofabrication Using a Combination of 3D Printing and Melt- Electrowriting of Shape-Morphing Polymers ACS Appl. Mater. & Interfaces 2021, 13 (11), 12767–12776
  Constante, G.; Apsite, I.; Alkhamis, H.; Dulle, M.; Schwarzer, M.; Caspari, A.; Synytska, A.; Salehi, S.; Ionov, L.
  
• Shape-morphing fibrous hydrogel/elastomer bilayers fabricated by a combination of 3D printing and melt electrowriting for muscle tissue regeneration ACS Appl. Bio Materials 2021, 4 (2), 1720–1730
  Uribe-Gomez, J.; Posada-Murcia, A.; Ergin. M.; Constante, G.; Apsite, I.; Schwarzer, M.; Caspari, A.; Synytska, A.; Salehi, S.; Ionov, L.