Molecular analysis of intestinal stem cell associated genes in colorectal cancer (CRC)
Final Report Abstract
Extensive functional analyses of the 3 candidate genes for colorectal tumor progression, IGF2, CITED4 and KLF6, confirmed their role in cellular processes that are associated with tumor development and dissemination. The most intriguing results were achieved by knockdown (IGF2, CITED4) or knockout (KLF6) of the respective candidate genes. The data confirmed the concept of a role in regulation of cell proliferation (IGF2, CITED4), cell adhesion (IGF2, KLF6) and migration (KLF6). Microarray based expression profiling subsequent to genetic manipulation of each of these candidate genes highlighted a further role of these genes in a number of unexpected cellular pathways and processes including cell/cell contacts (IGF2, CITED4) and the cytoskeleton (CITED4). Altogether, the expression profiles of the respective cell lines revealed a number of deregulated pathways that warrant further analysis in colorectal tumor models.
Publications
- CITED4 gene silencing in colorectal cancer cells modulates adherens/tight junction gene expression and reduces cell proliferation. J Cancer Res Clin Oncol. 2016 Jan;142(1):225-37
Rogers MA, Kalter V, Marcias G, Zapatka M, Barbus S, Lichter P
(See online at https://doi.org/10.1007/s00432-015-2011-5) - IGF2 knockdown in two colorectal cancer cell lines decreases survival, adhesion and modulates survival-associated genes. Tumor Biol. 2016 Sep;37(9):12485-12495
Rogers MA, Kalter V, Strowitzki M, Schneider M, Lichter P
(See online at https://doi.org/10.1007/s13277-016-5115-x)
