Impact of MFM disease mutations on the assembly mechanism and network formation of muscle-specific intermediate filament proteins
Zusammenfassung der Projektergebnisse
We have demonstrated the impact of point mutations in desmin on the assembly of the protein into filament networks and their interaction with large authentic structural muscle proteins such as nebulin and nebulette. Furthermore, we investigated the interaction of desmin with the IF proteins synemin-L and nestin identifying both proteins not to be authentic IF proteins, expected to form hetero-dimeric or hetero-tetrameric complexes with desmin. Instead, according to our results, these proteins should be classified as IF-associated proteins what may have important implications with respect to how we look at their incorporation into the differentiating muscle cell. Moreover, we investigated the interaction of both wild-type and mutated desmins with the small heat shock protein αB-crystallin/HSPB5. From these experiments it is clear that the chaperone does not only help to assemble unit-length filaments but also exhibits a sensor function during assembly, discriminating between wild-typ desmin filaments and filaments formed by mutated desmins. Accordingly, these results may explain why mutations in αB-crystallin/HSPB5 can lead to a complete reorganization of the desmin network in patient myocytes.
Projektbezogene Publikationen (Auswahl)
- Nebulin binding impedes mutant desmin filament assembly. Mol Biol Cell 2013; 24:1918-1932
Baker LK, Gillis DC, Sharma S, Ambrus A, Herrmann H, Conover GM
(Siehe online unter https://doi.org/10.1091/mbc.E12-11-0840) - Attractive interactions among intermediate filaments determine network mechanics in vitro. PLoS One 2014; 9:e93194
Pawelzyk P, Mücke N, Herrmann H, Willenbacher N
(Siehe online unter https://doi.org/10.1371/journal.pone.0093194) - Impact of ion valency on the assembly of vimentin studied by quantitative small angle X-ray scattering. Soft Matter 2014; 10: 2059-68
Brennich M, Bauch S, Vainio U, Wedig T, Herrmann H, Köster S
(Siehe online unter https://doi.org/10.1039/c3sm52532e) - Mechanosensing through focal adhesion-anchored intermediate filaments. FASEB J 2014; 28:715-729
Gregor M, Osmanagic-Myers S, Burgstaller G, Wolfram M, Fischer I, Walko G, Resch GP, Jörgl A, Herrmann H, Wiche G
(Siehe online unter https://doi.org/10.1096/fj.13-231829) - Microtubule-dependent transport of vimentin filament precursors is regulated by actin and the concerted action of Rho- and p21-activated kinases. FASEB J 2014; 28:2879-2890
Robert A, Herrmann H, Davidson MW, Gelfand VI
(Siehe online unter https://doi.org/10.1096/fj.14-250019) - Sequenceresolved free energy profiles of stress-bearing vimentin intermediate filaments. Proc Natl Acad Sci USA 2014; 111:11359-11364
Ramm B, Stigler J, Hinczewski M, Thirumalai D, Herrmann H, Woehlke G, Rief M
(Siehe online unter https://doi.org/10.1073/pnas.1403122111) - The toxic effect of R350P mutant desmin in striated muscle of man and mouse. Acta Neuropathol 2015; 129:297-315
Clemen CS, Stöckigt F, Strucksberg KH, Chevessier F, Winter L, Schütz J, Bauer R, Thorweihe JM, Wenzel D, Schneider-Stock R, Schlötzer-Schrehardt U, Rasche V, Krsmanovic P, Katus HA, Rottbauer W, Just S, Müller O, Friedrich O, Meyer R, Herrmann H, Schrickel JW, Schröder R
(Siehe online unter https://doi.org/10.1007/s00401-014-1363-2) - In vitro Assembly Kinetics of Cytoplasmic Intermediate Filaments: A Correlative Monte Carlo Simulation Study. PLoS One 2016; 11:e0157451
Mücke N, Winhein S, Merlitz H, Buchholz J, Langowski J, Herrmann H
(Siehe online unter https://doi.org/10.1371/journal.pone.0157451) - Nebulette is a powerful cytolinker organizing desmin and actin in mouse hearts. Mol Biol Cell 2016; 27:3869-3882
Hernandez DA, Bennett CM, Dunina-Barkovskaya L, Wedig T, Capetanaki Y, Herrmann H, Conover G
(Siehe online unter https://doi.org/10.1091/mbc.E16-04-0237) - Structural Dynamics of the Vimentin Coiled-Coil Contact Regions involved in Filament Assembly as revealed by Hydrogen-Deuterium Exchange. J Biol Chem 2016; 291:24931–24950
Premchandar A, Mücke N, Poznański J, Wedig T, Kaus-Drobek M, Herrmann H, Dadlez M
(Siehe online unter https://doi.org/10.1074/jbc.M116.748145) - The filament forming reactions of vimentin tetramers studied in a serial-inlet microflow device by small angle x-ray scattering. Biomicrofluidics 2016; 10:024108
Saldanha O, Brennich ME, Burghammer M, Herrmann H, Köster S
(Siehe online unter https://doi.org/10.1063/1.4943916) - B-crystallin is a sensor for assembly intermediates and for the subunit topology of desmin intermediate filaments. Cell Stress Chaperones 2017; 22:613-626
Sharma S, Conover GM, Elliott JL, Der Perng M, Herrmann H, Quinlan RA
(Siehe online unter https://doi.org/10.1007/s12192-017-0788-7)
