Effects of chloroplastic originated-H2O2 in signalling and biotic interactions
Final Report Abstract
In summary, we showed that H2O2 originating in a specific organelle induces two types of responses: one that integrates signals independently from the subcellular site of H2O2 production and another that is dependent of the H2O2 production site. H2O2 produced in peroxisomes induces transcripts involved in protein repair responses, while H2O2 produced in chloroplasts induces early signalling responses, including transcription factors and biosynthetic genes involved in production of secondary signalling messengers. The transcriptional dynamics of the TFs after H2O2 production was assessed and the analysis of subcellular localization and potential H2O2-triggered mobility of selected TFs was initiated. We showed induced resistance of plants producing H2O2 from the chloroplasts against C. higginsianum, which is completely dependent on WRKY33. We now hypothesize that indole carbonyl nitriles might play a role in defense against C. higginsianum and will work further in this direction. Our work on RCS indicated that detoxification of RCS involves at least three subcellular compartments and depends on a specific GLXI isoform during germination. Furthermore, our work allow to propose that GLXI;like proteins are not active GLX. Further work aiming to understand the molecular and biological function of GLXI-like proteins is in progress. We wrote a review article on our work on damage-control systems for small molecules, which includes ROS and RCS. Nine peer-reviewed manuscripts were published and new avenues of research in my group were opened. Six students obtained their PhD or Master degrees working on the project.
Publications
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Expression of ROS-responsive genes and transcription factors after metabolic formation of H2O2 in chloroplasts. Frontiers in Plant Science, 3.
Balazadeh, Salma; Jaspert, Nils; Arif, Muhammad; Mueller-Roeber, Bernd & Maurino, Veronica G.
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Spatial H2O2 Signaling Specificity: H2O2 from Chloroplasts and Peroxisomes Modulates the Plant Transcriptome Differentially. Molecular Plant, 7(7), 1191-1210.
Sewelam, Nasser; Jaspert, Nils; Van Der Kelen, Katrien; Tognetti, Vanesa B.; Schmitz, Jessica; Frerigmann, Henning; Stahl, Elia; Zeier, Jürgen; Van Breusegem, Frank & Maurino, Veronica G.
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Activation of cyclic electron flow by hydrogen peroxide in vivo. Proceedings of the National Academy of Sciences, 112(17), 5539-5544.
Strand, Deserah D.; Livingston, Aaron K.; Satoh-Cruz, Mio; Froehlich, John E.; Maurino, Veronica G. & Kramer, David M.
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Dissecting Long-Term Adjustments of Photoprotective and Photo-Oxidative Stress Acclimation Occurring in Dynamic Light Environments. Frontiers in Plant Science, 7.
Matsubara, Shizue; Schneider, Trang & Maurino, Veronica G.
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Defense against Reactive Carbonyl Species Involves at Least Three Subcellular Compartments Where Individual Components of the System Respond to Cellular Sugar Status. The Plant Cell, 29(12), 3234-3254.
Schmitz, Jessica; Dittmar, Isabell C.; Brockmann, Jörn D.; Schmidt, Marc; Hüdig, Meike; Rossoni, Alessandro W. & Maurino, Veronica G.
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Biochemical control systems for small molecule damage in plants. Plant Signaling & Behavior, 13(5), e1477906.
Hüdig, M.; Schmitz, J.; Engqvist, M. K. M. & Maurino, V. G.
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Dissecting the Physiological Function of Plant Glyoxalase I and Glyoxalase I-Like Proteins. Frontiers in Plant Science, 9.
Schmitz, Jessica; Rossoni, Alessandro W. & Maurino, Veronica G.
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Reactive oxygen species dosage in Arabidopsis chloroplasts can improve resistance towards Colletotrichum higginsianum by the induction of WRKY33. New Phytologist, 226(1), 189-204.
Schmidt, Andree; Mächtel, Rebecca; Ammon, Alexandra; Engelsdorf, Timo; Schmitz, Jessica; Maurino, Veronica G. & Voll, Lars M.
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The AtHSP17.4C1 Gene Expression Is Mediated by Diverse Signals that Link Biotic and Abiotic Stress Factors with ROS and Can Be a Useful Molecular Marker for Oxidative Stress. International Journal of Molecular Sciences, 20(13), 3201.
Sewelam, Nasser; Kazan, Kemal; Hüdig, Meike; Maurino, Veronica G. & Schenk, Peer M.
