Evolution von viridans Streptokokken von Menschen und Primaten: Genome und Gentransfer
Final Report Abstract
The comparison of viridans streptococci isolates from human and primates revealed several new aspects concerning the evolution of Streptococcus pneumoniae. The occurrence of S. oralis in primates, not only in animals held in captivity but also in free living chimpanzees documents that this species is part of the oral flora of these animals. S. mitis was also found among the isolates from great apes. All major genes encoding S. pneumoniae virulence factors not detected in previous analyses were now found in S. mitis and/or S. oralis. These data are in agreement with the assumption that S. pneumoniae is derived from an ancient S. mitis clone, and that diversification of the S. pneumoniae species has evolved as a consequence of the growing population of human. The expansion of genetic elements BOX and RUP in S. pneumoniae is likely to play an important role in this process. Several new Streptococcus species were found among the isolates obtained from primates. Only the three choline-binding proteins LytB required for cell separation and CbpD/CbpF important for genetic competence are conserved in S. pneumoniae, S. mitis and S. oralis. Consequently, genes involved in the biosynthesis of choline-containing teichoic acids are widespread among all three species. A biosynthesis cluster present in some isolates of S. mitis and S. oralis is distinct from that of S. pneumoniae and leads to a new lipoteichoic acid variant with distint structural features.
Publications
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2012. Biosynthesis of teichoic acids in Streptococcus pneumoniae and closely related species: lessons from genomes. Microb. Drug Res. 18: 344-358
Denapaite, D., Brückner, R., Hakenbeck, R. and W. Vollmer
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Characterization of choline independent transformant of S. pneumoniae. 3rd Symposium The dynamics of peptidoglycan structure and function: New insights into the Great Wall. Paris, France, September 23- 25, 2013
Denapaite D., Heß N., Sørensen U.B.S, Simorre J.-P., Hakenbeck R and Brückner R.
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Genetic relatedness of Streptococcus spp. isolated from human and apes. TRAP - Competence, Transformation and their Biological Consequences in the Pneumococcus, Kaiserslautern, August 19-22, 2013
Denapaite, D., Kappler, P., Leendertz, F., Ochigawa, I. and R. Hakenbeck
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Genetic Relatedness of Streptococcus spp. isolated from human and primates. Microbiology after the genomics revolution: Genomes 2014; Paris, June 24-27, 2014
Denapaite, D., Rieger, M., Köndgen, S., Leendertz, F. and R. Hakenbeck
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Genomics, genetic variation, and regions of differences. S. 81–107. In: Streptococcus pneumoniae: molecular mechanisms of host-pathogen interactions. Amsterdam, Academic Press/Elsevier (2015). Eds: Brown, J., Hammerschmidt, S. and C. Orihuela. 9780124105300
Tettelin H., Chancey S., Mitchell T., Denapaite D., Schähle, Y., Rieger, M. and R. Hakenbeck
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Lipoteichoic acid of Streptococcus oralis Uo5: a novel biochemical structure comprising an unusual phosphorylcholine substitution pattern compared to Streptococcus pneumoniae. Scientific Reports volume 5, Article number: 16718 (2015), 14 S.
Gisch, N., Schwudke, D., Thomsen, S., Heß N., Hakenbeck, R., and D. Denapaite
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New insights into structure, biosynthesis and pro-inflammatory potential of pneumococcal teichoic acids. EuroPneumo, Oxford, 2015
N. Gisch, T. P. Kohler, F. Waldow, D. Schwudke, H. Heine, R. Hakenbeck, D. Denapaite and S. Hammerschmidt
