Docking and recycling of secretory vesicles studied by high and super-resolution microscopy (P05)

• Subject Area: Biophysics
• Term: from 2011 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 180879236

Project P5 focuses on the organization and coupling/crosstalk of the two major membrane microcompartments during synaptic vesicle (SV) cycling in neurons, the sites of docking and fusion (exocytosis) of the so-called readily releasable pool (RRP) of SVs at the ‘active zone’, and the sites for SV retrieval (endocytosis) at the periactive zone. The group succeeded in formation of functional, purely presynaptic boutons onto glass coverslips, microstructured and functionalized with synaptic cell-adhesion molecules. They will use this new preparation to study aspects of the structure and function of exo- and endocytic microcompartments by super-resolution fluorescence microscopy and correlative SEM.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 944: Physiology and Dynamics of Cellular Microcompartments
• Applicant Institution: Universität Osnabrück
• Project Head: Professor Dr. Jürgen Klingauf