Gene regulation by alpha-hydroxyketone-mediated signaling in Legionella pneumophila
Zusammenfassung der Projektergebnisse
Legionella pneumophila, the causative agent of Legionnaires´ pneumonia, employs for cellcell communication the small signaling molecule LAI-1 (3-hydroxypentadecane-4-one), which is produced and detected by the Lqs (Legionella quorum sensing) system. The system comprises the autoinducer synthase LqsA, the homologous sensor kinases LqsS and LqsT, and the prototypic response regulator LqsR . Genetic, biochemical and cell biological approaches indicated that Lqs-regulated processes include pathogen-phagocyte interactions, production of extracellular filaments and biofilms, as well as natural competence for DNA uptake. We identified and characterized (1) the novel L. pneumophila sensor kinase LqsT, (2) phosphorylation signaling through LqsS and LqsT onto LqsR, (3) inter-kingdom signaling of LAI-1 through the eukaryotic components IQGAP1, Cdc42 and ARHGEF9, as well as (4) regulation of LqsS- and LqsT-dependent phosphorylation signaling and gene expression by LAI-1. The original aims (ii) function and structure of the response regulator LqsR, and (iii) endogenous production of LAI-1 by L. pneumophila are the focus of ongoing studies.
Projektbezogene Publikationen (Auswahl)
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(2012) α-Hydroxyketone production and sensing by Legionella and Vibrio. Sensors 12: 2899-2919
Tiaden, A. & Hilbi, H.
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(2013) Analysis of Legionella infection by flow cytometry. In Meth. Mol. Biol. 954, Legionella: methods and protocols. Buchrieser, C. & Hilbi, H. (eds.), Springer Press; pp. 233-249
Tiaden, A., Kessler, A. & Hilbi, H.
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(2013) Legionella: a versatile model to study host pathogen interactions. In Meth. Mol. Biol. 954, Legionella: methods and protocols. Buchrieser, C. & Hilbi, H. (eds.), Springer Press; editorial, pp. v-xix
Hilbi, H. & Buchrieser, C.
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(2013) The Legionella pneumophila orphan sensor kinase LqsT regulates competence and pathogen-host interactions as a component of the LAI-1 circuit. Environ. Microbiol. 15: 646-662
Kessler, A., Schell, U., Sahr, T., Tiaden, A., Harrison, C. F., Buchrieser, C. & Hilbi, H.
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(2014) Phosphorylation signaling through the Legionella quorum sensing histidine kinases LqsS and LqsT converges on the response regulator LqsR. Mol. Microbiol. 92: 1039–1055
Schell, U., Kessler, A. & Hilbi, H.
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(2014) The natural alternative: protozoa as cellular models for Legionella infection. Cell. Microbiol. 16: 15-26
Hoffmann, C., Harrison, C. F. & Hilbi, H.
