Elucidation of the unique conjugative DNA-translocation machinery of mycelial streptomycetes by biochemical analysis and real time imaging (A13)

• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2011 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 32152271

Streptomycetes developed a unique conjugative DNA-transfer system. In a process derived from the segregation of chromosomal DNA during bacterial cell division, a double-stranded DNA molecule is translocated and subsequently distributed to the mycelial compartments of the recipient. By reconstituting the DNA-transfer apparatus in vitro and by real time imaging of the DNA-transfer, we aim to elucidate the molecular processes directing Streptomyces conjugation.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 766: The Bacterial Cell Envelope: Structure, Function and Infection Interface
• Applicant Institution: Eberhard Karls Universität Tübingen
• Project Head: Dr. Günther Muth