Aufklärung der Ribosomen-Entstehung durch den Einsatz eines thermophilen Eukaryonten

• Antragsteller: Professor Dr. Eduard Christian Hurt
• Fachliche Zuordnung: Biochemie
• Förderung: Förderung von 2011 bis 2017
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 201179576

• Erstellungsjahr: 2018

Zusammenfassung der Projektergebnisse

After 6 years of experimental investigations within this Koselleck project, we could make substantial progress on every aspect of the Koselleck project initially described in the proposal. As anticipated, Chaetomium thermophilum ribosome biogenesis factors turned out to have in many aspects excellent properties for this type of research. Currently, many follow-up projects are going on in the laboratory, all based on findings derived from the Koselleck project.

Projektbezogene Publikationen (Auswahl)

• Structural basis for kinase-activity dependent placeholder role of Rio2 on the evolving pre-40S subunit. Nat. Struct. Mol. Biol. 19, 1316-1323 (2012)
  Ferreira-Cerca, Sébastien; Sagar, Vatsala; Schäfer, Thorsten; Diop, Momar; Wesseling, Anne-Maria; Lu, Haiyun; Chai, Eileen; Hurt, Ed & LaRonde-LeBlanc, Nicole
  
• Synchronizing Nuclear Import of Ribosomal Proteins with Ribosome Assembly. Science 338, 666-671 (2012)
  Kressler, Dieter; Bange, Gert; Ogawa, Yutaka; Stjepanovic, Goran; Bradatsch, Bettina; Pratte, Dagmar; Amlacher, Stefan; Strauß, Daniela; Yoneda, Yoshihiro; Katahira, Jun; Sinning, Irmgard & Hurt, Ed
  
• Consistent mutational paths predict eukaryotic thermostability. BMC Evolutionary Biology 13:7 (2013)
  van Noort, Vera; Bradatsch, Bettina; Arumugam, Manimozhiyan; Amlacher, Stefan; Bange, Gert; Creevey, Chris; Falk, Sebastian; Mende, Daniel R; Sinning, Irmgard; Hurt, Ed & Bork, Peer
  
• Coupled GTPase and remodeling ATPase activities form a checkpoint for ribosome export. Nature 505, 112-116 (2014)
  Matsuo, Yoshitaka; Granneman, Sander; Thoms, Matthias; Manikas, Rizos-Georgios; Tollervey, David & Hurt, Ed
  
• A network of assembly factors involved is involved in remodeling rRNA elements during pre-ribosome maturation. J. Cell Biol. 207, 481-98 (2014)
  Baßler, Jochen; Paternoga, Helge; Holdermann, Iris; Thoms, Matthias; Granneman, Sander; Barrio-Garcia, Clara; Nyarko, Afua; Lee, Woonghee; Stier, Gunter; Clark, Sarah A.; Schraivogel, Daniel; Kallas, Martina; Beckmann, Roland; Tollervey, David; Barbar, Elisar; Sinning, Irmi & Hurt, Ed
  
• An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum. Nucl. Acid Res. (2014)
  Bock, Thomas; Chen, Wei-Hua; Ori, Alessandro; Malik, Nayab; Silva-Martin, Noella; Huerta-Cepas, Jaime; Powell, Sean T.; Kastritis, Panagiotis L.; Smyshlyaev, Georgy; Vonkova, Ivana; Kirkpatrick, Joanna; Doerks, Tobias; Nesme, Leo; Baßler, Jochen; Kos, Martin; Hurt, Ed; Carlomagno, Teresa; Gavin, Anne-Claude; Barabas, Orsolya; ... & Bork, Peer
  
• Catalytic Rio1 kinase/ATPase mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes. Nucl. Acid Res. (2014)
  Ferreira-Cerca, Sébastien; Kiburu, Irene; Thomson, Emma; LaRonde, Nicole & Hurt, Ed
  
• Construction of the central protuberance requires rotation of 5S RNP during 60S ribosome biogenesis. Nature Com. 5, 3491 (2014)
  Leidig, Christoph; Thoms, Matthias; Holdermann, Iris; Bradatsch, Bettina; Berninghausen, Otto; Bange, Gert; Sinning, Irmgard; Hurt, Ed & Beckmann, Roland
  
• Concerted removal of the Erb1-Ytm1 complex in ribosome biogenesis relies on an elaborate interface. Nucl. Acid Res. 44, 926-39 (2016)
  Thoms, Matthias; Ahmed, Yasar Luqman; Maddi, Karthik; Hurt, Ed & Sinning, Irmgard
  
• Coordinated ribosomal L4 protein assembly into the pre-ribosome is regulated by its eukaryote-specific extension. Mol. Cell 58, 854-862 (2015)
  Stelter, Philipp; Huber, Ferdinand M.; Kunze, Ruth; Flemming, Dirk; Hoelz, André & Hurt, Ed
  
• Cotranslational capturing of nascent ribosomal proteins by their dedicated chaperones. Nat. Comm. 6, 7494 (2015)
  Pausch, Patrick; Singh, Ujjwala; Ahmed, Yasar Luqman; Pillet, Benjamin; Murat, Guillaume; Altegoer, Florian; Stier, Gunter; Thoms, Matthias; Hurt, Ed; Sinning, Irmgard; Bange, Gert & Kressler, Dieter
  
• Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site. Nature Com. 6:6510 (2015)
  Calviño, Fabiola R.; Kharde, Satyavati; Ori, Alessandro; Hendricks, Astrid; Wild, Klemens; Kressler, Dieter; Bange, Gert; Hurt, Ed; Beck, Martin & Sinning, Irmgard
  
• The exosome is recruited to RNA substrates through specific adapter proteins. Cell 162, 1029-1038 (2015)
  Thoms, Matthias; Thomson, Emma; Baßler, Jochen; Gnädig, Marén; Griesel, Sabine & Hurt, Ed
  
• Architecture of the 90S pre-ribosome: a structural view on the birth of the eukaryotic ribosome. Cell 166, 380–393 (2016)
  Kornprobst, Markus; Turk, Martin; Kellner, Nikola; Cheng, Jingdong; Flemming, Dirk; Koš-Braun, Isabelle; Koš, Martin; Thoms, Matthias; Berninghausen, Otto; Beckmann, Roland & Hurt, Ed
  
• Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies Sci Rep. 6, 20937 (2016)
  Kellner, Nikola; Schwarz, Johannes; Sturm, Miriam; Fernandez-Martinez, Javier; Griesel, Sabine; Zhang, Wenzhu; Chait, Brian T.; Rout, Michael P.; Kück, Ulrich & Hurt, Ed
  
• The K+-dependent GTPase Nug1 is implicated in the association of the helicase Dbp10 to the immature peptidyl transferase centre during ribosome maturation. Nucl Acid Res. 44, 1800-1812 (2016)
  Manikas, Rizos-Georgios; Thomson, Emma; Thoms, Matthias & Hurt, Ed
  
• 3.2 Angström resolution structure of the 90S pre-ribosome before A1 pre-rRNA cleavage. Nat. Struct. Mol. Biol. (2017)
  Cheng, Jingdong; Kellner, Nikola; Berninghausen, Otto; Hurt, Ed & Beckmann, Roland
  
• Interaction network of the ribosome assembly machinery from a eukaryotic thermophile. Protein Sci. 26, 327-342 (2017)
  Baßler, Jochen; Ahmed, Yasar Luqman; Kallas, Martina; Kornprobst, Markus; Calviño, Fabiola R.; Gnädig, Marén; Thoms, Matthias; Stier, Gunter; Ismail, Sherif; Kharde, Satyavati; Castillo, Nestor; Griesel, Sabine; Bastuck, Sonja; Bradatsch, Bettina; Thomson, Emma; Flemming, Dirk; Sinning, Irmgard & Hurt, Ed
  
• Interdependent action of KH domain proteins Krr1 and Dim2 drive the 40S platform assembly. Nat Commun. 20;8(1):2213 (2017)
  Sturm, Miriam; Cheng, Jingdong; Baßler, Jochen; Beckmann, Roland & Hurt, Ed
  
• Mpp10 represents a platform for the interaction of multiple factors from the 90S pre-ribosome. PLOS One 12 (2017)
  Sá-Moura, Bebiana; Kornprobst, Markus; Kharde, Satyavati; Ahmed, Yasar Luqman; Stier, Gunter; Kunze, Ruth; Sinning, Irmgard & Hurt, Ed
  
• Structural basis for 5’-ETS recognition by Utp4 at the early stages of ribosome biogenesis. PLOS One (2017)
  Calviño, Fabiola R.; Kornprobst, Markus; Schermann, Géza; Birkle, Fabienne; Wild, Klemens; Fischer, Tamas; Hurt, Ed; Ahmed, Yasar Luqman & Sinning, Irmgard
  
• Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes. Cell, 171(7):1599-1610.e14 (2017)
  Kater, Lukas; Thoms, Matthias; Barrio-Garcia, Clara; Cheng, Jingdong; Ismail, Sherif; Ahmed, Yasar Luqman; Bange, Gert; Kressler, Dieter; Berninghausen, Otto; Sinning, Irmgard; Hurt, Ed & Beckmann, Roland