Dynamic-mechanical cell manipulation and characterization using magnetostrain

• Applicants: Professor Dr. Stefan Mayr; Professorin Dr. Mareike Zink
• Subject Area: Statistical Physics, Nonlinear Dynamics, Complex Systems, Soft and Fluid Matter, Biological Physics
• Term: from 2012 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 201601437

• Final Report Year: 2024

Final Report Abstract

The present research project was centered around the idea of coupling living cells to a mechanical actuator with the aim of i) mechanically interacting with the cells and ii) detecting changes in the stress within the cell or the cell-actuator interfaces. While for the latter a cantilever-type of geometry had been proposed, the actuators employed were based on (magnetic) shape memory elements, which had just become available towards the beginning of the project. Besides utilizing these "smart metals", a shape memory effect in collagenous hydrogels, which was discovered in the course of this project, proved also be highly promising within this scope due to higher attainable strains. As the actuator-living matter interface proves to be of central importance for the results, significant effort was devoted for thorough characterization and optimization.

Publications

• Interaction of Ferromagnetic Shape Memory Alloys and RGD Peptides for Mechanical Coupling to Cells: from Ab Initio Calculations to Cell Studies. Advanced Functional Materials, 23(11), 1383-1391.
  Zink, Mareike; Szillat, Florian; Allenstein, Uta & Mayr, Stefan G.
  
• Fe–Pd based ferromagnetic shape memory actuators for medical applications: Biocompatibility, effect of surface roughness and protein coatings. Acta Biomaterialia, 9(3), 5845-5853.
  Allenstein, U.; Ma, Y.; Arabi-Hashemi, A.; Zink, M. & Mayr, S.G.
  
• Ferromagnetic shape memory alloys: Synthesis, characterisation and biocompatibility of Fe–Pd for mechanical coupling to cells. Materials Science and Technology, 30(13), 1579-1589.
  Zink, M. & Mayr, S. G.
  
• Interfacing hard and living matter: plasma-assembled proteins on inorganic functional materials for enhanced coupling to cells and tissue. J. Mater. Chem. B, 2(44), 7739-7746.
  Allenstein, U.; Szillat, F.; Weidt, A.; Zink, M. & Mayr, S. G.
  
• Contractile cell forces deform macroscopic cantilevers and quantify biomaterial performance. Soft Matter, 11(25), 5053-5059.
  Allenstein, U.; Mayr, S. G. & Zink, M.
  
• Coupling of Metals and Biominerals: Characterizing the Interface between Ferromagnetic Shape-Memory Alloys and Hydroxyapatite. ACS Applied Materials & Interfaces, 7(28), 15331-15338.
  Allenstein, Uta; Selle, Susanne; Tadsen, Meike; Patzig, Christian; Höche, Thomas; Zink, Mareike & Mayr, Stefan G.
  
• Ferromagnetic shape memory alloys: from ion-beam-assisted synthesis to plasmaaided functionalization for biomedical applications, DPG Spring Meeting, Berlin, March 2015
  A. Arabi-Hashemi; U. Allenstein; A Bischoff; I. Claussen; T. Edler; M. Fuchs; A. Landgraf; A. M. Jakob; N- John; L. Kühnemund; Y. Ma; M. Müller; F. Szillat; C. Vree; A. Weidt; M. Zink & S. G. Mayr
  
• Interfacing Living Cells and Fe-Pd Ferromagnetic Shape Memory Alloys: Experiments and Modeling on Different Functionalization Strategies, Yale School of Engineering & Applied Science, May 2015
  U. Allenstein & S. G. Mayr
  
• Intelligente Metalle und schaltbare Gummibärchen: Entwicklung neuartiger Materialien für die Medizin, MNU-Kongress Leipzig, March 2016
  M. Zink & S. G. Mayr
  
• Mechanical spectroscopy of retina explants at the protein level employing nanostructured scaffolds. Soft Matter, 12(14), 3431-3441.
  Mayazur, Rahman S.; Reichenbach, Andreas; Zink, Mareike & Mayr, Stefan G.
  
• Shock wave induced martensitic transformations and morphology changes in Fe-Pd ferromagnetic shape memory alloy thin films. Applied Physics Letters, 108(15).
  Bischoff, A. J.; Arabi-Hashemi, A.; Ehrhardt, M.; Lorenz, P.; Zimmer, K. & Mayr, S. G.
  
• Insights into Growth of Fe7Pd3 Ferromagnetic Shape Memory Alloy Thin Films. Crystal Growth & Design, 17(5), 2374-2378.
  Bischoff, A. J.; Hua, K. & Mayr, S. G.
  
• Micropatterning of reagent‐free, high energy crosslinked gelatin hydrogels for bioapplications. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 106(1), 320-330.
  Heyart, Benedikt; Weidt, Astrid; Wisotzki, Emilia I.; Zink, Mareike & Mayr, Stefan G.
  
• Programing stimuli-responsiveness of gelatin with electron beams: basic effects and development of a hydration-controlled biocompatible demonstrator. Scientific Reports, 7(1).
  Riedel, Stefanie; Heyart, Benedikt; Apel, Katharina S. & Mayr, Stefan G.
  
• Smart biomaterials and switchable systems for biomedical applications: Fundamentals, synthesis and applications, Kolloquium des SFB/TR 67, Leipzig, 2017
  S. G. Mayr
  
• Early adhesion of cells to ferromagnetic shape memory alloys functionalized with plasma assembled biomolecules – a single cell force spectroscopy study. Materials & Design, 158, 19-27.
  Cakir, Mehmet Volkan; Allenstein, Uta; Zink, Mareike & Mayr, Stefan G.
  
• Reagent-Free Programming of Shape-Memory Behavior in Gelatin by Electron Beams: Experiments and Modeling. Physical Review Applied, 9(2).
  Riedel, Stefanie & Mayr, Stefan G.
  
• Smart materials for biomedical applications: fundamental physics, synthesis, modeling and applications, Physik-Kolloquium, Universität Augsburg, Dec. 2018
  M. Deuflhard; J. Dietrich; J. Fella; S. Friebe; P. Friedrich; P. Hietschold; A. Holm; T. Isensee; U. Kapprell; J. Karoos; M. Mütze; S. Riedel; S. Schulze; F. Schütte; D. Warkotsch; A. Weidt; N. Wilharm & S. G. Mayr
  
• Intelligente biomimetische Oberflächen für medizinische Anwendungen, Vom Labor in die Praxis / Workshop, IHK Leipzig, Nov. 2019
  M. Deuflhard; J. Dietrich; S. Friebe; P. Friedrich; P. Hietschold; A. Holm; U. Kapprell; B. Klaus; M. Mütze; C. Pfeiffer; S. Riedel; F. Schütte; T. Slotosch; C. Watson; A. Weidt; N. Wilharm & S. G. Mayr
  
• Smart biomaterials and switchable systems: fundamentals, synthesis, modeling and applications, Universität Salzburg, Oct. 2019
  U. Allenstein; K. Apel; A. Arabi-Hashemi; J. Buchwald; M. Deuflhard; J. Dietrich; T. Edler; F. Engert; S. Friebe; P. Friedrich; M. Hennes; B. Heyart; P. Hietschold; A. Holm; S. Huth; A. Jakob; U. Kapprell; L. Kühnemund; B. Klaus; I. Kock; K. Krömmelbein; A. Landgraf; F. Lehnert; Y. Ma; M. Mütze; C. Pfeiffer; S. Rahman; S. Riedel; F. Schütte; T. Slotosch; C. Suckfüll; F. Szillat; M. Tadsen; C. Watson; A. Weidt; R. Werner; N. Wilharm; E. Wisotzki & S. G. Mayr
  
• Smart materials for biomedical applications: energetic-particle-assisted synthesis and functionalization, 21st international summer school on vacuum, electron and ion technologies (VEITH 2019), Sept. 2019
  M. Deuflhard; J. Dietrich; S. Friebe; P. Friedrich; P. Hietschold; A. Holm; U. Kapprell; B. Klaus; M. Mütze; C. Pfeiffer; S. Riedel; F. Schütte; T. Slotosch; C. Watson; A. Weidt; N. Wilharm & S. G. Mayr
  
• Structural properties and interface dynamics of Fe7Pd3 shape memory alloys in the martensitic phase: an ab-initio-based molecular dynamics study, Leibniz MMS Days 2019, Kühlungsborn, March 2019
  A. Holm & S. G. Mayr
  
• Energetic particle synthesized biomimetic surfaces, 7th International Plasma Science & Interfaces Workshop, Bochum, Nov. 2020
  M. Deuflhard; J. Dietrich; S. Friebe; P. Friedrich; P. Hietschold; A. Holm; U. Kapprell; B. Klaus; M. Mütze; C. Pfeiffer; S. Riedel; F. Schütte; T. Slotosch; C. Watson; A. Weidt; N. Wilharm & S. G. Mayr
  
• Ferromagnetic shape memory alloys: from ion-beam-assisted synthesis to plasma-aided functionalization for biomedical applications, Kolloquium, Universität Jena, June 2015
  A. Arabi-Hashemi; U. Allenstein; A. Bischoff; I. Claussen; T. Edler; M. Fuchs; A. Landgraf; A. M. Jakob; L. Kühnemund; Y. Ma; M. Müller; F. Szillat; C. Vree; A. Weidt; M. Zink & S. G. Mayr
  
• Oberflächen und Materialien für biomedizinische Anwendungen: Ein Überblick über Synthese und Funktionalisierung mit energetischen Partikeln, EFDS - FABM: Oberflächen und Beschichtungen in der Bio- und Medizintechnik (online), May 2020
  M. Deuflhard; J. Dietrich; S. Friebe; P. Friedrich; P. Hietschold; A. Holm; U. Kapprell; B. Klaus; M. Mütze; C. Pfeiffer; S. Riedel; F. Schütte; T. Slotosch; C. Watson; A. Weidt; N. Wilharm & S. G. Mayr
  
• Electron Beam-Treated Enzymatically Mineralized Gelatin Hydrogels for Bone Tissue Engineering. Journal of Functional Biomaterials, 12(4), 57.
  Riedel, Stefanie; Ward, Daniel; Kudláčková, Radmila; Mazur, Karolina; Bačáková, Lucie; Kerns, Jemma G.; Allinson, Sarah L.; Ashton, Lorna; Koniezcny, Robert; Mayr, Stefan G. & Douglas, Timothy E. L.
  
• Nanoporous Morphogenesis in Amorphous Carbon Layers: Experiments and Modeling on Energetic Ion Induced Self‐Organization. Advanced Theory and Simulations, 4(7).
  Hoffmann, Daniel T.; Dietrich, Johannes; Mändl, Stephan; Zink, Mareike & Mayr, Stefan G.
  
• Conductive Tracks in Carbon Implanted Titania Nanotubes: Atomic‐Scale Insights from Experimentally Based Ab Initio Molecular Dynamics Modeling. Advanced Theory and Simulations, 5(6).
  Holm, Alexander; Kupferer, Astrid; Mändl, Stephan; Lotnyk, Andriy & Mayr, Stefan G.