Struktur und Funktion einer bakteriellen Ionen (Na+)-translozierenden Ferredoxin: NAD+-Oxidoreduktase (Rnf)

• Antragsteller: Professor Dr. Volker Müller
• Fachliche Zuordnung: Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen
• Förderung: Förderung von 2011 bis 2021
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 206018227

• Erstellungsjahr: 2021

Zusammenfassung der Projektergebnisse

This project, the identification of the primary sodium ion pump in the acetogen A. woodii, started 20 years ago in 2001. In the beginning, we and others speculated that the primary pump is part of the WLP, possibly the methyltransferase, as in methanogenic archaea. However, research over the years disproved this hypothesis. After 10 years of chasing a ghost a new idea was born in a big melting point of ideas, data, genome sequences under contribution of Profs. Buckel, Gottschalk and Thauer. The exciting idea was that many anaerobes may have a respiratory ferredoxin:NAD oxidoreductase that, in acetogens, may channel electrons to the WLP enzymes. In the last ten years, we have provided a wealth of direct and indirect data published in many papers that the primary, Na+ translocating respiratory enzyme in A. woodii is the Rnf complex that catalyzes ferredoxin-dependent NAD reduction with concomittant Na+ export.

Projektbezogene Publikationen (Auswahl)

• (2018) The Rnf complex is an energy coupled transhydrogenase essential to reversibly link cellular NADH and ferredoxin pools in the acetogen Acetobacterium woodii. J. Bacteriol. 00357-18
  Westphal, L., Wiechmann, A., Baker, J., Minton, N.P., Müller, V.
  (Siehe online unter https://doi.org/10.1128/jb.00357-18)
• (2020) The Rnf complex from the acetogenic bacterium Acetobacterium woodii: Purification and characterization of RnfC and RnfB. Biochim. Biophys. Acta - Bioenerg. 1861 : 148263
  Kuhns, M., Schuchmann, V., Schmidt, S., Friedrich, T., Wiechmann, A., Müller, V.
  (Siehe online unter https://doi.org/10.1016/j.bbabio.2020.148263)
• (2020) The Rnf complex is a Na+ coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima. Commun. Biol. 3 : 431
  Kuhns, M., Trifunović, D., Huber, H., Müller, V.
  (Siehe online unter https://doi.org/10.1038/s42003-020-01158-y)
• (2021) Homologous production, one step purification and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuels. Biotechnol. Biofuels 13 : 208
  Wiechmann, A., Trifunović, D., Klein, S., Müller, V.
  (Siehe online unter https://doi.org/10.1186/s13068-020-01851-4)