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Projekt Druckansicht

Modulation of ABA signaling by Ca2+-dependent posttranslational regulation

Fachliche Zuordnung Zell- und Entwicklungsbiologie der Pflanzen
Förderung Förderung von 2011 bis 2019
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 210911670
 
Erstellungsjahr 2015

Zusammenfassung der Projektergebnisse

Aims of the DFG Project "Modulation of ABA signaling by Ca 2+-dependent posttranslational regulation” were to test the activation of SnRK2 kinases in ABA signaling by calcium dependent processes and to study the interaction of ABA-signaling with biotic signaling pathways. We could further corroborate the initial finding that CBL1 and CIPK1 are required for stomatal aperture regulation. We could furthermore show that SnRK2 kinases are not directly regulated by CIPK1 via phosphorylation but probably via an indirect mechanism. We identified downstream processes regulated by the activity of SnRK2 kinases, including the subnuclear localization of WRKY40 to PHYTOCHOME B-containing nuclear bodies. Upon ABA- treatment, WRKY40 protein relocalizes to the nucleoplasm within 15 minutes, which might pose a new posttranslational regulatory mechanism for WRKY transcription factors. Localization to PHYB- containing nuclear bodies might link ABA to light-signaling in seed germination. Following up on the role of WRKY transcription factors in ABA signaling, we pursued a reverse genetic screen and identified WRKY43 as a negative regulator of ABA-inhibition of seed germinate. In further genetic analyses we could show that WRKY43 regulates components of the central AFL-network in seed germination and influences lipid fatty acid composition which results in an increased abiotic stress tolerance of wrky43 seeds. Overall this project could show that calcium dependent processes have a pivotal role in ABA-signal transduction and that biotic signaling pathways and ABA-signaling are intricately linked not only in stomatal aperture regulation but also during seed germination.

Projektbezogene Publikationen (Auswahl)

 
 

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