Molekulare Charakterisierung von Genen mit gemeinsamen und unterschiedlichen Eigenschaften der Oct4 Regulation in pluripotenten Zellen
Zellbiologie
Zusammenfassung der Projektergebnisse
We finished all experiments planed to characterize the NF-Y complex that was proposed in our initial proposal. In addition, we extended the initially proposed in vitro experiments by in vivo characterization of the NF-Y complex. We generated Lap-tagged NF-Ya mice and performed a panel of in vivo experiment to investigate its functions. For goal II, we finished all experiments scheduled, and developed a novel protein level dependency (PLD) strategy to investigate the post-transcriptional regulation. We generated a panel of 36 BAC-tagged ES cell lines and employed them for subcellular localization and PLD assay. The PLD approach should find broad applicability in system biology as it opens a systematic way to study posttranscriptional regulation in cells after perturbations.
Projektbezogene Publikationen (Auswahl)
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(2013) “Quantitative interaction screen of telomeric repeat-containing RNA reveals novel TERRA regulators.”, Genome Res. 2013 Dec;23(12):2149-57
Scheibe, Arnoult, Kappei, Buchholz, Decottignies, Butter, Mann
