Molecular characterization of genes with shared and diverse attributes of Oct4 regulation in pluripotent cells

• Applicant: Professor Dr. Frank Buchholz
• Subject Area: Developmental Biology ; Cell Biology
• Term: from 2012 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 214450084

• Final Report Year: 2015

Final Report Abstract

We finished all experiments planed to characterize the NF-Y complex that was proposed in our initial proposal. In addition, we extended the initially proposed in vitro experiments by in vivo characterization of the NF-Y complex. We generated Lap-tagged NF-Ya mice and performed a panel of in vivo experiment to investigate its functions. For goal II, we finished all experiments scheduled, and developed a novel protein level dependency (PLD) strategy to investigate the post-transcriptional regulation. We generated a panel of 36 BAC-tagged ES cell lines and employed them for subcellular localization and PLD assay. The PLD approach should find broad applicability in system biology as it opens a systematic way to study posttranscriptional regulation in cells after perturbations.

Publications

• (2013) “Quantitative interaction screen of telomeric repeat-containing RNA reveals novel TERRA regulators.”, Genome Res. 2013 Dec;23(12):2149-57
  Scheibe, Arnoult, Kappei, Buchholz, Decottignies, Butter, Mann