Charakterisierung der Rolle von Lypd6 im Wnt/ß-Catenin-Signalweg
Final Report Abstract
The Wnt/beta-catenin signaling pathway represents one of the ways in which cells communicate with each other. The pathway carries out a myriad of essential functions during embryonic development of vertebrates, in the maintenance and control of stem cells in adult animals, and in the regeneration of tissues. If misregulated, it can lead to a variety of diseases, particularly cancer. A complete understanding of the regulation of this signaling pathway is therefore of great importance. In this project, we described a new function of a previously poorly understood protein, Lypd6, and discovered that it regulates the Wnt signaling pathway. Specifically, Lypd6 enhances the response of cells to Wnt signals. Through experiments in zebrafish embryos and mammalian cell cultures we were able to show that Lypd6 is localized in the plasma membrane of cells and ensures that the Wnt co-receptor LRP6 can be fully activated in response to Wnt ligands. This happens by Lypd6 recruiting the co-receptor into specific membrane domains, the so-called “lipid rafts”.
Publications
-
Lypd6 Enhances Wnt/β-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains. Developmental Cell, 26(4), 331-345.
Özhan, Günes; Sezgin, Erdinc; Wehner, Daniel; Pfister, Astrid S.; Kühl, Susanne J.; Kagermeier-Schenk, Birgit; Kühl, Michael; Schwille, Petra & Weidinger, Gilbert
-
Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments. The FEBS Journal, 284(15), 2513-2526.
Sezgin, Erdinc; Azbazdar, Yagmur; Ng, Xue W.; Teh, Cathleen; Simons, Kai; Weidinger, Gilbert; Wohland, Thorsten; Eggeling, Christian & Ozhan, Gunes
