Analysis of Enabled (Ena) and GUK-holder (GUKh) in regulating WAVE-driven actin dynamics in Drosophila

• Antragsteller: Professor Dr. Sven Bogdan
• Fachliche Zuordnung: Entwicklungsbiologie
• Förderung: Förderung von 2012 bis 2019
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 219587464

• Erstellungsjahr: 2019

Zusammenfassung der Projektergebnisse

Epithelial morphogenesis require a coordinated interplay between membrane receptors and the cytoskeleton. Our data uncovered a mechanism by which polarity information can be transduced from a membrane receptor to a key actin regulator to control collective follicle cell migration during egg elongation. 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechanism driving rotational motions in epithelial morphogenesis. Together with Klaus Ebnet group we will further follow up these observations in MCF10A cell lines with inducible expression of shRNAs targeting the adhesion receptors and the actin regulators.

Projektbezogene Publikationen (Auswahl)

• (2016) Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. J. Cell Biol. 212: 591-603
  Squarr A.J., Brinkmann K., Chen B., Steinbacher T., Ebnet K., Rosen M.K. and Bogdan, S.
  (Siehe online unter https://doi.org/10.1083/jcb.201508081)
• (2016) WHAMY is a novel actin polymerase promoting myoblast fusion, macrophage cell motility and sensory organ development in Drosophila. J Cell Sci. 129: 604-620
  Brinkmann K., Winterhoff M., Onel, S.F., Schultz J., Faix J. and Bogdan S.
  (Siehe online unter https://doi.org/10.1242/jcs.179325)
• (2017). Adherens Junctions on the Move-Membrane Trafficking of E- Cadherin. Cold Spring Harb Perspect Biol 9
  Brüser L, Bogdan S
  (Siehe online unter https://doi.org/10.1101/cshperspect.a029140)
• (2017). Molecular Control of Actin Dynamics In Vivo: Insights from Drosophila. Handbook of experimental pharmacology 235: 285-310
  Brüser L, Bogdan S.
  (Siehe online unter https://doi.org/https://doi.org/10.1007/164_2016_33)
• (2018). Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo. Methods Molecular Biology Vol. 1749, 978-1-4939
  Rüder M, Nagel BM, Bogdan S
  (Siehe online unter https://doi.org/10.1007/978-1-4939-7701-7_17)
• (2019). Transient localization of the Arp2/3 complex initiates neuronal dendrite branching in vivo. Development. Apr 4;146(7)
  Stuerner, T., Tatarnikova, A., Mueller, J., Schaffran, B., Cuntz, H., Zhang, Y., Nemetova, M., Bogdan, S., Small, V. and Tavosanis, G.
  (Siehe online unter https://doi.org/10.1242/dev.171397)