Analysis of Enabled (Ena) and GUK-holder (GUKh) in regulating WAVE-driven actin dynamics in Drosophila

• Applicant: Professor Dr. Sven Bogdan
• Subject Area: Developmental Biology
• Term: from 2012 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 219587464

• Final Report Year: 2019

Final Report Abstract

Epithelial morphogenesis require a coordinated interplay between membrane receptors and the cytoskeleton. Our data uncovered a mechanism by which polarity information can be transduced from a membrane receptor to a key actin regulator to control collective follicle cell migration during egg elongation. 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechanism driving rotational motions in epithelial morphogenesis. Together with Klaus Ebnet group we will further follow up these observations in MCF10A cell lines with inducible expression of shRNAs targeting the adhesion receptors and the actin regulators.

Publications

• (2016) Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. J. Cell Biol. 212: 591-603
  Squarr A.J., Brinkmann K., Chen B., Steinbacher T., Ebnet K., Rosen M.K. and Bogdan, S.
  (See online at https://doi.org/10.1083/jcb.201508081)
• (2016) WHAMY is a novel actin polymerase promoting myoblast fusion, macrophage cell motility and sensory organ development in Drosophila. J Cell Sci. 129: 604-620
  Brinkmann K., Winterhoff M., Onel, S.F., Schultz J., Faix J. and Bogdan S.
  (See online at https://doi.org/10.1242/jcs.179325)
• (2017). Adherens Junctions on the Move-Membrane Trafficking of E- Cadherin. Cold Spring Harb Perspect Biol 9
  Brüser L, Bogdan S
  (See online at https://doi.org/10.1101/cshperspect.a029140)
• (2017). Molecular Control of Actin Dynamics In Vivo: Insights from Drosophila. Handbook of experimental pharmacology 235: 285-310
  Brüser L, Bogdan S.
  (See online at https://doi.org/https://doi.org/10.1007/164_2016_33)
• (2018). Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo. Methods Molecular Biology Vol. 1749, 978-1-4939
  Rüder M, Nagel BM, Bogdan S
  (See online at https://doi.org/10.1007/978-1-4939-7701-7_17)
• (2019). Transient localization of the Arp2/3 complex initiates neuronal dendrite branching in vivo. Development. Apr 4;146(7)
  Stuerner, T., Tatarnikova, A., Mueller, J., Schaffran, B., Cuntz, H., Zhang, Y., Nemetova, M., Bogdan, S., Small, V. and Tavosanis, G.
  (See online at https://doi.org/10.1242/dev.171397)