Cellular Logistics - Traceless protein modification by small high-affinity tags in living cells

• Applicant: Professor Dr. Robert Tampé
• Subject Area: Biochemistry ; Biological and Biomimetic Chemistry
• Term: from 2012 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 223458327

Labeling and modification of intracellular proteins remain a major challenge in life sciences, often limited by large fusion domains, high quantities of costly probes, and long reaction times. Here, we will guide protein trans-splicing by minimal high-affinity interaction pairs to trace proteins´ function and assembly in living cells. In addition, we aim at modulating cellular processes by light with high resolution in time and space. Combined with super-resolution microscopy and single-molecule analysis, these traceless approaches shall provide valuable insights into the co-operative function and suprastructures of proteins in particular at intracellular membranes.

• DFG Programme: Priority Programmes
• Subproject of: SPP 1623: Chemoselective Reactions for the Synthesis and Application of Functional Proteins