Genetically modified pigs to modulate vascular and cellular rejection (B02)

• Subject Area: Animal Breeding, Animal Nutrition, Animal Husbandry ; Immunology
• Term: from 2012 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213602983

We have produced single loci multi-modified pigs to combat hyperacute and vascular rejection. Our goal is now to improve long-term xenograft survival. We will a) define the multi-transgene integration site and determine PERV load; b) characterise transgene expression patterns in detail and assess the survival and functionality of multi-modified porcine tissues and organs as xenografts; c) introduce additional modifications as necessary; d) develop a "smart graft" strategy to express immuno-regulatory and tissue-protective transgenes specifically during phases of rejection.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 127: Biology of Xenogeneic Cell and Organ Transplantation - From Bench to Bedside
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Heads: Tatiana Flisikowska, Ph.D.; Professor Dr. Percy Alexander Knolle; Professorin Dr. Angelika Schnieke