Time-optimized NMR spectroscopy for the investigation of challenging biological systems

• Applicant: Dr. Manuel Etzkorn
• Subject Area: Structural Biology
• Term: from 2013 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 228568119

• Final Report Year: 2021

No abstract available

Publications

• Oriented Membrane Protein Reconstitution into Tethered Lipid Membranes for AFM Force Spectroscopy. Biophysical Journal 2016, 111, 1925
  Schmid, Sonja; Götz, Markus & Hugel, Thorsten
  
• Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization. Angewandte Chemie Int. Ed. 2016, 55, 10746
  Viennet, Thibault; Viegas, Aldino; Kuepper, Arne; Arens, Sabine; Gelev, Vladimir; Petrov, Ognyan; Grossmann, Tom N.; Heise, Henrike & Etzkorn, Manuel
  
• The power, pitfalls and potential of the nanodiscs system for NMR-based studies. Biological Chemistry 2016, 397, 1335
  Viegas, Aldino; Viennet, Thibault & Etzkorn, Manuel
  
• UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection. Journal of Biomolecular NMR 2016, 64, 9
  Viegas, Aldino; Viennet, Thibault; Yu, Tsyr-Yan; Schumann, Frank; Bermel, Wolfgang; Wagner, Gerhard & Etzkorn, Manuel
  
• DNP-Enhanced MAS NMR: A Tool to Snapshot Conformational Ensembles of α-Synuclein in Different States. Biophysical Journal 2018, 114, 1614
  Uluca, Boran; Viennet, Thibault; Petrović, Dušan; Shaykhalishahi, Hamed; Weirich, Franziska; Gönülalan, Ayşenur; Strodel, Birgit; Etzkorn, Manuel; Hoyer, Wolfgang & Heise, Henrike
  
• Structural insights from lipid-bilayer nanodiscs link α-Synuclein membrane-binding modes to amyloid fibril formation. Communications Biology 2018, 1, 44
  Viennet, Thibault; Wördehoff, Michael M.; Uluca, Boran; Poojari, Chetan; Shaykhalishahi, Hamed; Willbold, Dieter; Strodel, Birgit; Heise, Henrike; Buell, Alexander K.; Hoyer, Wolfgang & Etzkorn, Manuel
  
• Reconstitution and NMR characterization of the ion-channel accessory subunit barttin in detergents and lipid-bilayer nanodiscs. Frontiers in Molecular Biosciences 2019, 6, 13
  Viennet, Thibault; Bungert-Plümke, Stefanie; Elter, Shantha; Viegas, Aldino; Fahlke, Christoph & Etzkorn, Manuel
  
• α-synuclein-derived lipoparticles in the study of α-Synuclein amyloid fibril formation. Chemistry and Physics of Lipids 2019, 220, 57
  Falke, Marcel; Victor, Julian; Wördehoff, Michael M.; Peduzzo, Alessia; Zhang, Tao; Schröder, Gunnar F.; Buell, Alexander K.; Hoyer, Wolfgang & Etzkorn, Manuel
  
• Influence of monovalent metal ions on metal binding and catalytic activity of the 10–23 DNAzyme. Biological Chemistry 2021, 402, 99
  Rosenbach, Hannah; Borggräfe, Jan; Victor, Julian; Wuebben, Christine; Schiemann, Olav; Hoyer, Wolfgang; Steger, Gerhard; Etzkorn, Manuel & Span, Ingrid
  
• Molecular Architecture of a Network of Potential Intracellular EGFR Modulators: ARNO, CaM, Phospholipids, and the Juxtamembrane. Segment Structure 2020, 28, 54
  Viegas, Aldino; Yin, Dongsheng M.; Borggräfe, Jan; Viennet, Thibault; Falke, Marcel; Schmitz, Anton; Famulok, Michael & Etzkorn, Manuel
  
• Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation. Scientific Reports 2020, 10, 3578
  Viegas, Aldino; Dollinger, Peter; Verma, Neha; Kubiak, Jakub; Viennet, Thibault; Seidel, Claus A. M.; Gohlke, Holger; Etzkorn, Manuel; Kovacic, Filip & Jaeger, Karl-Erich
  
• Local deuteration enables NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems. Angewandte Chemie Int. Ed. 2021
  Dubey, Abhinav; Stoyanov, Nikolay; Viennet, Thibault; Chhabra, Sandeep; Elter, Shantha; Borggräfe, Jan; Viegas, Aldino; Nowak, Radosław P.; Burdzhiev, Nikola; Petrov, Ognyan; Fischer, Eric S.; Etzkorn, Manuel; Gelev, Vladimir & Arthanari, Haribabu