Time-optimized NMR spectroscopy for the investigation of challenging biological systems

• Applicant: Dr. Manuel Etzkorn
• Subject Area: Structural Biology
• Term: from 2013 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 228568119

• Final Report Year: 2021

Final Report Abstract

No abstract available

Publications

• Oriented Membrane Protein Reconstitution into Tethered Lipid Membranes for AFM Force Spectroscopy. Biophysical Journal 2016, 111, 1925
  Bronder AM, Bieker A, Elter S, Etzkorn M, Häussinger D, Oesterhelt F
  (See online at https://doi.org/10.1016/j.bpj.2016.08.023)
• Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization. Angewandte Chemie Int. Ed. 2016, 55, 10746
  Viennet T, Viegas A, Kuepper A, Arens S, Gelev V, Petrov O, Grossmann T, Heise H, Etzkorn M
  (See online at https://doi.org/10.1002/ange.201603205)
• The power, pitfalls and potential of the nanodiscs system for NMR-based studies. Biological Chemistry 2016, 397, 1335
  Viegas A, Viennet T, Etzkorn M
  (See online at https://doi.org/10.1515/hsz-2016-0224)
• UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection. Journal of Biomolecular NMR 2016, 64, 9
  Viegas A, Viennet T, Yu TY, Schumann F, Bermel W, Wagner G, Etzkorn M
  (See online at https://doi.org/10.1007/s10858-015-0008-7)
• DNP-Enhanced MAS NMR: A Tool to Snapshot Conformational Ensembles of α-Synuclein in Different States. Biophysical Journal 2018, 114, 1614
  Uluca B, Viennet T, Petrovic D, Shaykhalishahi H, Weirich F, Gönülalan A, Strodel B, Etzkorn M, Hoyer W, Heise H
  (See online at https://doi.org/10.1016/j.bpj.2018.02.011)
• Structural insights from lipid-bilayer nanodiscs link α-Synuclein membrane-binding modes to amyloid fibril formation. Communications Biology 2018, 1, 44
  Viennet T, Wördehoff MW, Uluca B, Poojari C, Shaykhalishahi H, Willbold D, Strodel B, Heise H, Buell AK, Hoyer W, Etzkorn M
  (See online at https://doi.org/10.1038/s42003-018-0049-z)
• Reconstitution and NMR characterization of the ion-channel accessory subunit barttin in detergents and lipid-bilayer nanodiscs. Frontiers in Molecular Biosciences 2019, 6, 13
  Viennet T, Bungert-Pümke S, Elter S, Viegas A, Fahlke C, Etzkorn M
  (See online at https://doi.org/10.3389/fmolb.2019.00013)
• α-synuclein-derived lipoparticles in the study of α-Synuclein amyloid fibril formation. Chemistry and Physics of Lipids 2019, 220, 57
  Falke M, Victor J, Wördehof M, Peduzzo A, Zhang T, Schröder G, Buell A, Hoyer W, Etzkorn M
  (See online at https://doi.org/10.1016/j.chemphyslip.2019.02.009)
• Molecular Architecture of a Network of Potential Intracellular EGFR Modulators: ARNO, CaM, Phospholipids, and the Juxtamembrane. Segment Structure 2020, 28, 54
  Viegas A, Yin DM, Borggräfe J, Viennet T, Falke M, Schmitz A, Famulok M, Etzkorn M
  (See online at https://doi.org/10.1016/j.str.2019.11.001)
• Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation. Scientific Reports 2020, 10, 3578
  Viegas A, Dollinger P, Verma N, Kubiak J, Viennet T, Seidel CAM, Gohlke H, Etzkorn M, Kovacic F, Jaeger KE
  (See online at https://doi.org/10.1038/s41598-020-60093-4)
• Influence of monovalent metal ions on metal binding and catalytic activity of the 10–23 DNAzyme. Biological Chemistry 2021, 402, 99
  Rosenbach H, Borggräfe J, Victor J, Wuebben C, Schiemann O, Hoyer W, Steger G, Etzkorn M, Span I
  (See online at https://doi.org/10.1515/hsz-2020-0207)
• Local deuteration enables NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems. Angewandte Chemie Int. Ed. 2021
  Dubey A, Stoyanov N, Viennet T, Chhabra S, Elter S, Borggräfe J, Viegas A, Nowak R, Burdzhiev N, Petrov O, Fischer ES, Etzkorn M, Gelev V, Arthanari H
  (See online at https://doi.org/10.1002/anie.202016070)