Chemoenzymatic synthesis of defined sulfated oligohyaluronans for binding studies and the construction of artificial extracellular matrices (A08)

• Subject Area: Public Health, Healthcare Research, Social and Occupational Medicine ; Biophysics
• Term: from 2013 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 59307082

J. Rademann and J. Schiller investigate the structure-activity relationship of defined sulfated oligohyaluronans as ligands of mediators and building blocks of aECM. For this purpose, a collection of defined sulfated hyaluronan (HA) -Tetra, hexa and octasaccharides as well as fluorescence and spin-labeled HA conjugates will employed in binding assays, structural- and functional analysis and immobilized onto surfaces. These GAG-derivatives will be analyzed by multidimensional mass spectrometry, ion mobility MS and pulsed field gradient NMR.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 67: Functional Biomaterials for Controlling Healing Processes in Bone und Skin - From Material Science to Clinical Application
• Applicant Institution: Universität Leipzig
• Project Heads: Professor Dr. Jörg Rademann; Privatdozent Dr. Jürgen Schiller