Dissecting Signaling Complexes and Dynamic Changes of the B-Cell (C02)

• Subject Area: Immunology
• Term: from 2013 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 215346292

Project C02 has established a portfolio of quantitative mass spectrometry (MS)-based proteomics methods for studying the proteome, phosphoproteome and specific protein interaction networks of B cells. In the second funding period, we comprehensively studied the interactomes of the inhibitory receptors CD22 and Siglec-G as well as the B cell activating factor receptor, BAFFR, and the Fc fragment of IgG receptor IIb. To explore B-cell signaling networks, a new quantitative phosphoproteomics approach was implemented and then employed to elucidate the PKC signaling network in B cells. Label-free proteomics was used for quantitative studies of (primary) B cell and plasma cell proteomes. In the new funding period, multi-conditional quantitative proteomics studies in B cells will be performed to delineate dynamic changes in B-cell-specific protein interactomes, phosphoproteomes and (primary) B cell populations. C02 will enable high-end quantitative proteomics studies in numerous projects and is therefore of central importance for the TRR130.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 130: B Cells: Immunity and Autoimmunity
• Applicant Institution: Friedrich-Alexander-Universität Erlangen-Nürnberg
• Project Head: Professorin Dr. Bettina Warscheid